Phiri Masauso Moses, Mulder Danielle Wingrove, Vorster Barend Christiaan
Centre for Human Metabolomics, North-West University, P/Bag X6001, Hoffman Street, Potchefstroom 2520, South Africa.
R Soc Open Sci. 2019 Feb 20;6(2):181971. doi: 10.1098/rsos.181971. eCollection 2019 Feb.
Gold nanostars (AuNSs) are seen as promising building blocks for biosensors with potential for easy readouts based on naked-eye and ultraviolet-visible spectroscopy detection. We present a seedless synthesis strategy for AuNSs that has the advantages of the seeded methods. The method used ascorbic acid as a reducing agent and silver nitrate as an anisotropic growth control assisting agent. AuNSs with multiple branches and a diameter of 59 nm were produced. They showed good stability when capped with PVP and modified with an enzyme in relatively strong ionic conditions. We investigated their application in plasmonic sensing by modifying them with glucose oxidase and detection of glucose. The AuNSs were found to be a good scaffold for the enzyme, proved to be stable and sensitive as transducers. Thus, the AuNSs showed good promise for further applications in plasmonic biosensing for biomedical diagnosis.
金纳米星（AuNSs）被视为生物传感器的理想构建模块，具有基于肉眼和紫外可见光谱检测实现轻松读数的潜力。我们提出了一种无籽合成策略来制备AuNSs，该策略具有种子法的优点。该方法使用抗坏血酸作为还原剂，硝酸银作为各向异性生长控制辅助剂。制备出了具有多个分支且直径为59 nm的AuNSs。当用聚乙烯吡咯烷酮（PVP）封端并在相对较强的离子条件下用酶进行修饰时，它们表现出良好的稳定性。我们通过用葡萄糖氧化酶修饰AuNSs并检测葡萄糖来研究它们在等离子体传感中的应用。发现AuNSs是该酶的良好支架，被证明作为传感器稳定且灵敏。因此，AuNSs在用于生物医学诊断的等离子体生物传感的进一步应用中显示出良好的前景。
