Delwatta Shehani L, Gunatilake Mangala, Baumans Vera, Seneviratne Melanie D, Dissanayaka Manjula L B, Batagoda Siyani S, Udagedara Asanga H, Walpola Prasad B
Faculty of Medical Sciences University of Sri Jayawardanepura Gangodawila Nugegoda Sri Lanka.
Faculty of Medicine University of Colombo Colombo Sri Lanka.
Animal Model Exp Med. 2018 Nov 21;1(4):250-254. doi: 10.1002/ame2.12041. eCollection 2018 Dec.
Lack of available reference values in a research setting under local conditions can be a drawback for beginners, as the accuracy of data from control samples cannot be checked at the beginning of a research project. This affects comparisons with data from test samples. To avoid these complications in their research projects, beginners tend to have a greater number of animals in the control group compared to test groups in order to have control group measurements within 2 SDs of the mean.
As non-availability of reference values was a long-felt need, the described project was conducted in order to establish a reference database for selected haematological, biochemical and physiological parameters using apparently healthy Sprague-Dawley rats bred in the Animal House of Faculty of Medicine, University of Colombo (UCFM).
Differences in mean values of packed cell volume (PCV), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), serum creatinine and blood glucose levels between the two genders were statistically significant. Lipid profile measurements did not differ significantly between genders, but mean and median values of triglycerides (TG) between male and female rats showed a difference of more than 10 mg/dL. The liver enzymes alkaline phosphatase (AP) and aspartate aminotransferase (AST) were also statistically significantly different between sexes. Despite wide variation in mean alanine aminotransferase (ALT) between sexes, the difference was not statistically significant.
The findings of this project should support to a certain extent the "Reduction" aspect of the 3Rs concept of Russell and Burch by reducing the number of Sprague-Dawley rats used in future research projects at UCFM.
在当地条件下的研究环境中缺乏可用的参考值，这可能对初学者来说是一个不利因素，因为在研究项目开始时无法检查对照样本数据的准确性。这会影响与测试样本数据的比较。为了避免研究项目中出现这些复杂情况，与测试组相比，初学者往往在对照组中使用更多数量的动物，以便使对照组的测量值在平均值的2个标准差范围内。
由于长期以来一直需要参考值，因此开展了所述项目，以便使用科伦坡大学医学院动物房饲养的表面健康的斯普拉格-道利大鼠，建立选定血液学、生化和生理参数的参考数据库。
两种性别之间的红细胞压积（PCV）、平均红细胞体积（MCV）和平均红细胞血红蛋白浓度（MCHC）、血清肌酐和血糖水平的平均值存在统计学显著差异。血脂谱测量在性别之间没有显著差异，但雄性和雌性大鼠甘油三酯（TG）的平均值和中位数显示相差超过10mg/dL。肝脏酶碱性磷酸酶（AP）和天冬氨酸转氨酶（AST）在性别之间也存在统计学显著差异。尽管两性之间丙氨酸转氨酶（ALT）的平均值差异很大，但差异无统计学意义。
本项目的研究结果应在一定程度上支持罗素和伯奇3R概念中的“减少”方面，即减少科伦坡大学医学院未来研究项目中使用的斯普拉格-道利大鼠数量。
