小麦胚tRNAArg的一级结构——精氨酰-tRNAArg：蛋白质转移酶的底物

The primary structure of wheat germ tRNAArg--the substrate for arginyl-tRNAArg:protein transferase.

作者信息

Barciszewska M Z, Keith G, Kubli E, Barciszewski J

出版信息

Biochimie. 1986 Mar;68(2):319-23. doi: 10.1016/s0300-9084(86)80029-3.

Abstract

Besides its major role in protein synthesis, wheat germ arginyl-tRNAArg can serve as an amino acid donor in an enzymatic reaction to bovine serum albumin catalysed by the enzyme arginyl-tRNAArg: protein transferase. The nucleotide sequence of the tRNAArg involved in this reaction was determined to be: pG-A-C-U-C-C-G-U-m1G-m2G-C-C-C-A-A-D-Gm-G-A-X-A-A-G-G-C-m2(2) G-C-U-G-G-U-Cm-U-I-C-G-m2A-A-A-C-C-A-G-A-G-A-D-U-m5C-U-G-G-G-T-psi -C-G-m1 A-U-C-C-C-C-A-G-C-G-G-A-G-U-C-G-C-C-AOH. We suggest that the decapentanucleotide 5'-G-U-Pu-m2G-C-N-C-A-A-D-Gm-G-A-X-A-3', localized in the D-region, interacts specifically with the protein transferase.

摘要

除了在蛋白质合成中发挥主要作用外，小麦胚芽精氨酰 - tRNAArg还可以在精氨酰 - tRNAArg：蛋白质转移酶催化的与牛血清白蛋白的酶促反应中作为氨基酸供体。确定参与该反应的tRNAArg的核苷酸序列为：pG - A - C - U - C - C - G - U - m1G - m2G - C - C - C - A - A - D - Gm - G - A - X - A - A - G - G - C - m2(2)G - C - U - G - G - U - Cm - U - I - C - G - m2A - A - A - C - C - A - G - A - G - A - D - U - m5C - U - G - G - G - T - ψ - C - G - m1A - U - C - C - C - C - A - G - C - G - G - A - G - U - C - G - C - C - AOH。我们认为位于D区域的十五核苷酸5'-G - U - Pu - m2G - C - N - C - A - A - D - Gm - G - A - X - A - 3'与蛋白质转移酶发生特异性相互作用。