Division of WCU (World Class University) Multiscale Mechanical Design, School of Mechanical and Aerospace Engineering, Seoul National University, Seoul, Republic of Korea.
Biofabrication. 2019 Apr 26;11(3):035013. doi: 10.1088/1758-5090/ab1402.
Microfluidics have enabled a wide range of experimental possibilities in the field of neuroscience. Unfortunately, the wider scale adoption of polydimethylsiloxane (PDMS) based microfluidic devices faces challenges due to inherent material compatibility issues and lack of standardized manufacturable devices. In this work, we present an injection molded plastic array three-dimensional (3D) neuron culture platform (Neuro-IMPACT) made of polystyrene (PS) with a standard 96-well plate form factor that can recapitulate elements of both the central and peripheral nervous systems. A standardized in vitro platform for neuron culture will facilitate the development of new therapies for neurodegenerative diseases, as they would enable quantitative analysis based on imaging as well as biochemical analysis. To demonstrate the versatility of Neuro-IMPACT, we modeled physiologically relevant complex co-culture models such as a 3D neuronal network, blood-brain barrier, and myelination. The Neuro-IMPACT offers a high-throughput screening compatible platform with the ability to engineer the neuronal microenvironment to aid both basic and applied neuroscience research.
微流控技术在神经科学领域中实现了广泛的实验可能性。然而，由于聚二甲基硅氧烷（PDMS）基微流控器件固有的材料兼容性问题和缺乏标准化可制造的器件，其更广泛的应用面临挑战。在这项工作中，我们提出了一种由聚苯乙烯（PS）制成的注塑成型塑料阵列三维（3D）神经元培养平台（Neuro-IMPACT），其具有标准的 96 孔板形式因子，可以再现中枢和外周神经系统的元素。神经元培养的标准化体外平台将促进神经退行性疾病新疗法的发展，因为它们可以基于成像和生化分析进行定量分析。为了展示 Neuro-IMPACT 的多功能性，我们模拟了生理相关的复杂共培养模型，如 3D 神经元网络、血脑屏障和髓鞘形成。Neuro-IMPACT 提供了一种高通量筛选兼容的平台，具有工程化神经元微环境的能力，有助于基础和应用神经科学研究。
