Prime S S, Malamos D, Rosser T, Scully C
J Oral Pathol. 1986 May;15(5):280-3. doi: 10.1111/j.1600-0714.1986.tb00624.x.
Oral epithelial atypia and foci of acantholytic dyskeratosis (FAD) were investigated in 54 rats treated with the carcinogen 0.5% (w/v) 4-nitroquinoline-N-oxide in propylene glycol and in 18 rats treated with propylene glycol only. The palate of each animal was painted 3 times weekly for up to 9 months and rats were killed at monthly intervals. A gradual significant increase in the epithelial atypia indices of the palatal and lingual tissues (anterior and posterior of the intermolar tubercle) was observed with a maximum value of 17-22 of a possible 75 at 28-32 weeks. No significant differences were noted between the atypia indices of the palatal and lingual tissues. FAD were not evident in the palate or lingual tissues before 12 weeks and 16-24 weeks, respectively, and although the palate consistently showed more FAD compared with the lingual tissues the differences were not significant. Features of epithelial atypia and FAD were absent in the 18 control rats treated with propylene glycol only and in 8 untreated control animals. At 28 weeks of 4NQO treatment 2 of 5 rats, at 32 weeks 3 of 4 rats and at 36 weeks 3 of 3 rats had developed infiltrating squamous cell carcinomas in either/both the palate or tongue. The results suggest that epithelial dysplasia and acantholytic dyskeratosis may be late morphological features of a more fundamental change occurring earlier in the process of tumour development.
对54只经丙二醇中0.5%（重量/体积）4-硝基喹啉-N-氧化物致癌物处理的大鼠以及18只仅用丙二醇处理的大鼠的口腔上皮异型增生和棘层松解性角化不良灶（FAD）进行了研究。每周给每只动物的腭部涂抹3次，持续9个月，大鼠每隔一个月处死。观察到腭部和舌部组织（磨牙间结节前后）的上皮异型增生指数逐渐显著增加，在28 - 32周时，在可能的75分中最大值为17 - 22分。腭部和舌部组织的异型增生指数之间未发现显著差异。FAD分别在12周和16 - 24周之前在腭部或舌部组织中不明显，尽管与舌部组织相比，腭部始终显示出更多的FAD，但差异不显著。仅用丙二醇处理的18只对照大鼠和8只未处理的对照动物中未出现上皮异型增生和FAD的特征。在4NQO处理28周时，5只大鼠中有2只，32周时4只大鼠中有3只，36周时3只大鼠中有3只在腭部或舌部出现了浸润性鳞状细胞癌。结果表明，上皮发育异常和棘层松解性角化不良可能是肿瘤发生过程中早期发生的更基本变化的晚期形态学特征。
