Oba Shino, Inaba Yohei, Shibuya Tomoyuki, Oshima Joji, Seyama Kuniaki, Kobayashi Toshihiro, Kunugita Naoki, Ino Toshihiro
Graduate School of Health Sciences, Gunma University, Maebashi, Gunma 371-8514, Japan.
Department of Health Promotion, National Institute of Public Health, Wako, Saitama 351-0197, Japan.
Exp Ther Med. 2019 Apr;17(4):2757-2764. doi: 10.3892/etm.2019.7252. Epub 2019 Feb 7.
Although several experimental studies have reported that oxidative stress levels decrease during smoking cessation, how they change among general smokers has yet to be completely elucidated. In the present study, a total of 23 smokers who underwent smoking cessation treatment were observed for two-week changes in their levels of 8-OHdG and 8-isoprostane. Physical and nutritional characteristics were measured at the initial patient visit, and casual urine samples were collected at the initial visit and at a follow-up visit two weeks later. Oxidative stress was measured by a high performance liquid chromatography electrochemical detector, and the two-week difference in the levels of oxidative stress was assessed according to demographic and nutrient factors. Neither the urinary level of 8-OHdG nor that of 8-isoprostane decreased, although the cotinine level was decreased at two weeks. A Two-way repeated ANOVA revealed a significant interaction for fat intake by time for the change in the 8-OHdG level (P=0.03) and significant interactions for α-tocopherol intake (P=0.03), iron intake, and carbohydrate intake (P=0.03), all of which were time-dependent for the change in the 8-isoprostane level. The 8-OHdG level decreased among smokers with a high fat intake and was increased with a low fat intake. The 8-isoprostane levels were decreased among smokers with a high carbohydrate intake and increased with a low carbohydrate intake, decreased with a low iron intake and increased with a high iron intake and decreased with a low α-tocopherol intake and increased with a high α-tocopherol intake. Although the present study failed to observe a decrease in oxidative stress levels during the two-week smoking cessation period, we hypothesize that the intake levels of specific nutrients when initiating smoking cessation treatment may predict any subsequent changes in the oxidative stress levels.
尽管多项实验研究报告称，戒烟期间氧化应激水平会降低，但普通吸烟者体内的氧化应激水平如何变化尚未完全阐明。在本研究中，共观察了23名接受戒烟治疗的吸烟者8-OHdG和8-异前列腺素水平在两周内的变化。在患者初次就诊时测量其身体和营养特征，并在初次就诊时和两周后的随访时收集随机尿样。通过高效液相色谱电化学检测器测量氧化应激，并根据人口统计学和营养因素评估氧化应激水平在两周内的差异。尽管两周时可替宁水平降低，但8-OHdG和8-异前列腺素的尿水平均未降低。双向重复方差分析显示，脂肪摄入量随时间变化对8-OHdG水平变化有显著交互作用（P = 0.03），α-生育酚摄入量（P = 0.03）、铁摄入量和碳水化合物摄入量随时间变化对8-异前列腺素水平变化也有显著交互作用。高脂肪摄入量的吸烟者8-OHdG水平降低，低脂肪摄入量者则升高。高碳水化合物摄入量的吸烟者8-异前列腺素水平降低，低碳水化合物摄入量者则升高；低铁摄入量者8-异前列腺素水平降低，高铁摄入量者则升高；低α-生育酚摄入量者降低，高α-生育酚摄入量者升高。尽管本研究未能观察到戒烟两周期间氧化应激水平降低，但我们推测，开始戒烟治疗时特定营养素的摄入水平可能预示氧化应激水平随后的任何变化。
