Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310000, P.R. China.
Department of Oncology, Guang Xing Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou, Zhejiang 310007, P.R. China.
Biosci Rep. 2019 Apr 26;39(4). doi: 10.1042/BSR20182057. Print 2019 Apr 30.
To investigate the significance of stanniocalcin-2 (STC2) expression in hepatocellular carcinoma (HCC) tissues and adjacent tissues. Levels of STC2 in HCC tissue were detected in 200 HCC patients tissues and adjacent tissues as controls by immunohistochemistry technique (IHC) and reverse transcriptase-PCR (RT-PCR). Single factor analysis was used to study the relationship between expression of STC2 mRNA and protein and clinicopathological features of HCC. Multifactor Cox survival analysis was used to relationship between the expression of STC2 and overall survival of postoperative patients with HCC. IHC staining showed that the expression of STC2 protein rate was 81.00% (163/200). And the positive rate of adjacent tissues was 29.00% (58/200). Western blot showed that the expression of STC2 protein in HCC was significantly higher than that in the adjacent tissues (<0.05). RT-PCR showed that the positive rates of STC2 mRNA expression in HCC were 75.50% (151/200), which was significantly higher than that in adjacent tissues 14.50% (29/200) (<0.05). Both STC2 mRNA and protein expression are related to tumor diameter, stage, tumor metastasis, carcinoma emboli in the portal vein and the degree of tumor differentiation in HCC. The HCC patients with higher expression of STC2 had shorter median survival time. STC2 expression, tumor diameter, carcinoma emboli in the portal vein, tumor differentiation degree, and tumor stage were independent factors affecting the overall survival of postoperative patients. The high expression of STC2 mRNA and protein expression in HCC may be associated with the occurrence, development, and prognosis of HCC. STC2 may also be possible to help developing new therapeutic strategies for HCC.
为了研究在肝细胞癌（HCC）组织和相邻组织中，Stanniocalcin-2（STC2）表达的意义。采用免疫组织化学技术（IHC）和逆转录-聚合酶链反应（RT-PCR）检测 200 例 HCC 患者组织和对照组织中 HCC 组织中 STC2 的水平。采用单因素分析研究 STC2 mRNA 和蛋白的表达与 HCC 临床病理特征的关系。多因素 Cox 生存分析用于研究 STC2 表达与术后 HCC 患者总生存率的关系。IHC 染色显示 STC2 蛋白的表达率为 81.00％（163/200）。相邻组织的阳性率为 29.00％（58/200）。Western blot 显示 HCC 中 STC2 蛋白的表达明显高于相邻组织（<0.05）。RT-PCR 显示 HCC 中 STC2 mRNA 表达的阳性率为 75.50％（151/200），明显高于相邻组织的 14.50％（29/200）（<0.05）。STC2 mRNA 和蛋白的表达均与 HCC 中的肿瘤直径、分期、肿瘤转移、门静脉癌栓和肿瘤分化程度有关。STC2 表达较高的 HCC 患者中位生存时间较短。STC2 表达、肿瘤直径、门静脉癌栓、肿瘤分化程度和肿瘤分期是影响术后患者总生存率的独立因素。HCC 中 STC2 mRNA 和蛋白表达较高可能与 HCC 的发生、发展和预后有关。STC2 也可能有助于为 HCC 开发新的治疗策略。
