Kucińska-Lipka Justyna, Gubanska Iga, Korchynskyi Olexandr, Malysheva Khrystyna, Kostrzewa Marcin, Włodarczyk Damian, Karczewski Jakub, Janik Helena
Department of Polymer Technology, Faculty of Chemistry, Gdank University of Technology, Narutowicza St. 11/12, 80-233 Gdansk, Poland.
Institute of Cell Biology, National Academy Science of Ukraine, 14/16 Drahomanov Str., 79005 Lviv, Ukraine.
Polymers (Basel). 2017 Aug 1;9(8):329. doi: 10.3390/polym9080329.
In this paper we describe the synthesis of poly(ester ether urethane)s (PEEURs) by using selected raw materials to reach a biocompatible polyurethane (PU) for biomedical applications. PEEURs were synthesized by using aliphatic 1,6-hexamethylene diisocyanate (HDI), poly(ethylene glycol) (PEG), α,ω-dihydroxy(ethylene-butylene adipate) (Polios), 1,4-butanediol (BDO) as a chain extender and calcium glycerolphosphate salt (GPCa) as a modifier used to stimulate bone tissue regeneration. The obtained unmodified (PURs) and modified with GPCa (PURs-M) PEEURs were studied by various techniques. It was confirmed that urethane prepolymer reacts with GPCa modifier. Further analysis of the obtained PURs and PURs-M by Fourier transform infrared (FTIR) and Raman spectroscopy revealed the chemical composition typical for PUs by the confirmed presence of urethane bonds. Moreover, the FTIR and Raman spectra indicated that GPCa was incorporated into the main PU chain at least at one-side. The scanning electron microscopy (SEM) analysis of the PURs-M surface was in good agreement with the FTIR and Raman analysis due to the fact that inclusions were observed only at 20% of its surface, which were related to the non-reacted GPCa enclosed in the PUR matrix as filler. Further studies of hydrophilicity, mechanical properties, biocompatibility, short term-interactions, and calcification study lead to the final conclusion that the obtained PURs-M may by suitable candidate material for further scaffold fabrication. Scaffolds were prepared by the solvent casting/particulate leaching technique (SC/PL) combined with thermally-induced phase separation (TIPS). Such porous scaffolds had satisfactory pore sizes (36⁻100 μm) and porosity (77⁻82%) so as to be considered as suitable templates for bone tissue regeneration.
在本文中，我们描述了通过使用选定的原材料来合成聚（酯醚聚氨酯）（PEEURs），以获得用于生物医学应用的生物相容性聚氨酯（PU）。PEEURs是通过使用脂肪族1,6 - 六亚甲基二异氰酸酯（HDI）、聚乙二醇（PEG）、α,ω - 二羟基（乙 - 丁二醇己二酸酯）（Polios）、1,4 - 丁二醇（BDO）作为扩链剂以及甘油磷酸钙盐（GPCa）作为用于刺激骨组织再生的改性剂来合成的。通过各种技术对获得的未改性（PURs）和用GPCa改性的（PURs - M）PEEURs进行了研究。证实了聚氨酯预聚物与GPCa改性剂发生反应。通过傅里叶变换红外（FTIR）和拉曼光谱对获得的PURs和PURs - M进行的进一步分析揭示了由于确认存在聚氨酯键而具有的PU典型化学组成。此外，FTIR和拉曼光谱表明GPCa至少在一侧被并入到PU主链中。PURs - M表面的扫描电子显微镜（SEM）分析与FTIR和拉曼分析结果高度一致，因为仅在其20%的表面观察到夹杂物，这些夹杂物与作为填料包裹在PUR基质中的未反应GPCa有关。对亲水性、机械性能、生物相容性、短期相互作用和钙化研究的进一步研究得出最终结论，即所获得的PURs - M可能是用于进一步支架制造的合适候选材料。通过溶剂浇铸/颗粒沥滤技术（SC/PL）结合热致相分离（TIPS）制备了支架。这种多孔支架具有令人满意的孔径（36⁻100μm）和孔隙率（77⁻82%），因此被认为是骨组织再生的合适模板。
