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GmSYP24，一个假定的突触融合蛋白基因，赋予了植物耐渗透/干旱、盐胁迫和 ABA 信号途径的能力。

GmSYP24, a putative syntaxin gene, confers osmotic/drought, salt stress tolerances and ABA signal pathway.

机构信息

Key Laboratory of Oil Crop Biology, Ministry of Agriculture, Wuhan, 430062, China.

Oil Crops Research Institute of Chinese Academy of Agriculture Sciences, Wuhan, 430062, China.

出版信息

Sci Rep. 2019 Apr 12;9(1):5990. doi: 10.1038/s41598-019-42332-5.

DOI:10.1038/s41598-019-42332-5
PMID:30979945
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6461667/
Abstract

As major environment factors, drought or high salinity affect crop growth, development and yield. Transgenic approach is an effective way to improve abiotic stress tolerance of crops. In this study, we comparatively analyzed gene structures, genome location, and the evolution of syntaxin proteins containing late embryogenesis abundant (LEA2) domain. GmSYP24 was identified as a dehydration-responsive gene. Our study showed that the GmSYP24 protein was located on the cell membrane. The overexpression of GmSYP24 (GmSYP24ox) in soybean and heteroexpression of GmSYP24 (GmSYP24hx) in Arabidopsis exhibited insensitivity to osmotic/drought and high salinity. However, wild type soybean, Arabidopsis, and the mutant of GmSYP24 homologous gene of Arabidopsis were sensitive to the stresses. Under the abiotic stresses, transgenic soybean plants had greater water content and higher activities of POD, SOD compared with non-transgenic controls. And the leaf stomatal density and opening were reduced in transgenic Arabidopsis. The sensitivity to ABA was decreased during seed germination of GmSYP24ox and GmSYP24hx. GmSYP24hx induced up-regulation of ABA-responsive genes. GmSYP24ox alters the expression of some aquaporins under osmotic/drought, salt, or ABA treatment. These results demonstrated that GmSYP24 played an important role in osmotic/drought or salt tolerance in ABA signal pathway.

摘要

作为主要的环境因素，干旱或高盐度会影响作物的生长、发育和产量。转基因方法是提高作物非生物胁迫耐受性的有效途径。在这项研究中，我们比较分析了含有晚期胚胎丰富（LEA2）域的突触蛋白的基因结构、基因组位置和进化。鉴定出 GmSYP24 是一个对脱水响应的基因。我们的研究表明，GmSYP24 蛋白位于细胞膜上。大豆中超表达 GmSYP24（GmSYP24ox）和拟南芥中异源表达 GmSYP24（GmSYP24hx）都表现出对渗透/干旱和高盐的不敏感性。然而，野生型大豆、拟南芥和拟南芥 GmSYP24 同源基因的突变体对这些胁迫敏感。在非生物胁迫下，与非转基因对照相比，转基因大豆植株具有更高的含水量和更高的 POD、SOD 活性。并且转基因拟南芥的叶片气孔密度和开度减小。GmSYP24ox 和 GmSYP24hx 在种子萌发过程中对 ABA 的敏感性降低。GmSYP24hx 诱导 ABA 响应基因的上调。GmSYP24ox 在渗透/干旱、盐或 ABA 处理下改变一些水通道蛋白的表达。这些结果表明，GmSYP24 在 ABA 信号通路中对渗透/干旱或耐盐性起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/441f1b976221/41598_2019_42332_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/9ef066a9b8f4/41598_2019_42332_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/8d8c4b2cf809/41598_2019_42332_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/f1fa9fe0aecb/41598_2019_42332_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/4b5457cc182f/41598_2019_42332_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/3f09f2f864a1/41598_2019_42332_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/b7fe690f94df/41598_2019_42332_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/441f1b976221/41598_2019_42332_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/9ef066a9b8f4/41598_2019_42332_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/8d8c4b2cf809/41598_2019_42332_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/f1fa9fe0aecb/41598_2019_42332_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/4b5457cc182f/41598_2019_42332_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/3f09f2f864a1/41598_2019_42332_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/b7fe690f94df/41598_2019_42332_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9598/6461667/441f1b976221/41598_2019_42332_Fig7_HTML.jpg

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