National Institute of Abiotic Stress Management, Baramati, Pune, Maharashtra, 413115, India.
National Bureau of Plant Genetic Resources, Regional Station, Srinagar, Jammu & Kashmir, 191132, India.
Plant Mol Biol. 2022 Sep;110(1-2):199-218. doi: 10.1007/s11103-022-01295-4. Epub 2022 Jul 2.
This study focused on enhancing resilience of soybean crops to drought and salinity stresses by overexpression of GmFAD3A gene, which plays an important role in modulating membrane fluidity and ultimately influence plants response to various abiotic stresses. Fatty acid desaturases (FADs) are a class of enzymes that mediate desaturation of fatty acids by introducing double bonds. They play an important role in modulating membrane fluidity in response to various abiotic stresses. However, a comprehensive analysis of GmFAD3 in drought and salinity stress tolerance in soybean is lacking. We used bean pod mottle virus (BPMV)-based vector for achieving rapid and efficient overexpression as well as silencing of Omega-3 Fatty Acid Desaturase gene from Glycine max (GmFAD3) to assess the functional role of GmFAD3 in abiotic stress responses in soybean. Higher levels of recombinant BPMV-GmFAD3A transcripts were detected in overexpressing soybean plants. Overexpression of GmFAD3A in soybean resulted in increased levels of jasmonic acid and higher expression of GmWRKY54 as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants under drought and salinity stress conditions. The GmFAD3A-overexpressing plants showed higher levels of chlorophyll content, efficient photosystem-II, relative water content, transpiration rate, stomatal conductance, proline content and also cooler canopy under drought and salinity stress conditions as compared to mock-inoculated, vector-infected and FAD3-silenced soybean plants. Results from the current study revealed that GmFAD3A-overexpressing soybean plants exhibited tolerance to drought and salinity stresses. However, soybean plants silenced for GmFAD3 were vulnerable to drought and salinity stresses.
本研究通过过表达 GmFAD3A 基因来提高大豆作物对干旱和盐胁迫的抗性，该基因在调节膜流动性方面起着重要作用，最终影响植物对各种非生物胁迫的响应。脂肪酸去饱和酶（FADs）是一类通过引入双键来介导脂肪酸去饱和的酶。它们在调节膜流动性以响应各种非生物胁迫方面起着重要作用。然而，对大豆中 GmFAD3 在干旱和盐胁迫耐受性方面的综合分析还很缺乏。我们使用豆荚斑驳病毒（BPMV）为基础的载体来实现快速有效的过表达以及沉默 Glycine max 中的 Omega-3 脂肪酸去饱和酶基因（GmFAD3），以评估 GmFAD3 在大豆非生物胁迫响应中的功能作用。在过表达大豆植物中检测到更高水平的重组 BPMV-GmFAD3A 转录物。与模拟接种、载体感染和 FAD3 沉默的大豆植物相比，在干旱和盐胁迫条件下，大豆中 GmFAD3A 的过表达导致茉莉酸水平升高，GmWRKY54 的表达更高。与模拟接种、载体感染和 FAD3 沉默的大豆植物相比，GmFAD3A 过表达的植株在干旱和盐胁迫条件下表现出更高的叶绿素含量、高效的光系统 II、相对含水量、蒸腾速率、气孔导度、脯氨酸含量，以及更凉爽的冠层。本研究的结果表明，GmFAD3A 过表达的大豆植株对干旱和盐胁迫具有耐受性。然而，沉默 GmFAD3 的大豆植株对干旱和盐胁迫敏感。
