College of Life Sciences and Oceanography Shenzhen University China.
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Optoelectronic Engineering Shenzhen University China.
FEBS Open Bio. 2019 Feb 21;9(4):629-642. doi: 10.1002/2211-5463.12596. eCollection 2019 Apr.
The transcription factor family plays a critical role in the ethylene signaling pathway, which regulates a broad spectrum of plant growth and developmental processes, as well as defenses to myriad stresses. Although genome-wide analysis of this family has been carried out for several plant species, no comprehensive analysis of the gene family in soybean has been reported so far. Furthermore, there are few studies on the functions of genes in soybean. In this study, we identified 12 soybean () genes, which we divided into three groups based on their phylogenetic relationships. We then detected their duplication status and found that most of the genes have duplicated copies derived from two whole-genome duplication events. These duplicated genes underwent strong negative selection during evolution. We further analyzed the transcript profiles of genes using the transcriptome data and found that their spatio-temporal and stress expression patterns varied considerably. For example, - were found to be strongly expressed in almost every sample, while - exhibited low expression, or were not expressed at all. Additionally, these genes showed different responses to dehydration, salinity and phosphate starvation. Finally, we surveyed genetic variations of these genes in 302 resequenced wild soybeans, landraces and improved soybean cultivars. Our data showed that most genes are well conserved, and are not modified in domesticated or improved cultivars. Together, these findings provide a potentially valuable resource for characterizing the gene family and lay the basis for further elucidation of their molecular mechanisms.
转录因子家族在乙烯信号通路中起着关键作用，该通路调节广泛的植物生长和发育过程，以及对多种胁迫的防御。尽管已经对几种植物物种进行了该家族的全基因组分析，但迄今为止尚未有关于大豆基因家族的全面分析。此外，关于大豆基因的功能研究也很少。在这项研究中，我们鉴定了 12 个大豆基因（），根据它们的系统发育关系将其分为三组。然后，我们检测了它们的重复状态，发现大多数基因都有来自两次全基因组复制事件的重复拷贝。这些重复基因在进化过程中经历了强烈的负选择。我们进一步利用转录组数据分析了基因的转录谱，发现它们的时空和应激表达模式有很大差异。例如，-在几乎每个样本中都强烈表达，而-表达水平较低，或者根本不表达。此外，这些基因对脱水、盐胁迫和磷饥饿表现出不同的反应。最后，我们在 302 个重测序的野生大豆、地方品种和改良大豆品种中调查了这些基因的遗传变异。我们的数据表明，大多数基因都很好地保守，在驯化或改良品种中没有修饰。总之，这些发现为研究基因家族提供了有价值的资源，并为进一步阐明其分子机制奠定了基础。
