DNA 折纸在病毒衣壳中的包装。

Packaging of DNA origami in viral capsids.

机构信息

Geza Ad Ltd, Givatayim 53251, Israel.

出版信息

Nanoscale. 2019 May 30;11(21):10160-10166. doi: 10.1039/c8nr10113b.

Abstract

Here we show the encapsulation of 35 nm diameter, nearly-spherical, DNA origami by self-assembly of SV40-like (simian virus 40) particles. The self-assembly of this new type of nanoparticles is highly reproducible and efficient. The structure of these particles was determined by cryo-EM. The capsid forms a regular SV40 lattice of T = 7d icosahedral symmetry and the structural features of encapsulated DNA origami are fully visible. These particles are a promising biomaterial for use in various medical applications.

摘要

在这里，我们展示了通过 SV40 样（猴病毒 40）颗粒的自组装来封装 35nm 直径的近球形 DNA 折纸。这种新型纳米颗粒的自组装具有高度的重现性和高效性。通过 cryo-EM 确定了这些颗粒的结构。衣壳形成了规则的 SV40 晶格，具有 T=7d 二十面体的对称性，封装的 DNA 折纸的结构特征完全可见。这些颗粒是一种很有前途的生物材料，可用于各种医疗应用。