Chotphruethipong Lalita, Benjakul Soottawat, Kijroongrojana Kongkarn
Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla 90112 Thailand.
J Food Sci Technol. 2019 Apr;56(4):1785-1792. doi: 10.1007/s13197-019-03617-9. Epub 2019 Feb 13.
Optimization of ultrasound-assisted extraction (UAE) of antioxidative phenolics from the leaves of cashew ( L.) was carried out by response surface methodology along with the central composite design. Two independent variables were considered: amplitude (30-77%) and time (7-31 min). The highest extraction yield was 23.61% when the optimal extraction condition (77% amplitude for 31 min) was implemented. The extract containing total phenolic content of 579.55 mg GAE/g dry extract possessed radical scavenging activities and reducing power. The experiment values were in line with the predicted counterparts. Extract contained gallic acid, isoquercetin, tannic acid, quercetin, catechin, apigenin, hydroquinin, eriodictyol, and rutin. The extract with increasing levels inhibited AAPH-induced DNA damage to a higher extent. Thus, UAE was demonstrated to potentially increase the extraction efficacy of phenolics from cashew leaves and the extract could be applied as a natural antioxidant.
采用响应面法结合中心复合设计对腰果（Anacardium occidentale L.）叶中抗氧化酚类物质的超声辅助提取（UAE）进行了优化。研究了两个自变量：振幅（30 - 77%）和时间（7 - 31分钟）。当采用最佳提取条件（77%振幅，31分钟）时，最高提取率为23.61%。提取物中总酚含量为579.55 mg GAE/g干提取物，具有自由基清除活性和还原能力。实验值与预测值相符。提取物含有没食子酸、异槲皮苷、单宁酸、槲皮素、儿茶素、芹菜素、对苯二酚、圣草酚和芦丁。随着提取物浓度的增加，其对AAPH诱导的DNA损伤的抑制作用增强。因此，超声辅助提取被证明有可能提高腰果叶中酚类物质的提取效率，该提取物可作为天然抗氧化剂应用。
