基于固相核磁共振分析的免疫吸附剂的开发。
Development of an Immunosorbent for Solid-Phase NMR-Based Assay.
作者信息
Khramtsov P V, Kropaneva M D, Bochkova M S, Timganova V P, Zamorina S A, Rayev M B
机构信息
Institute of Ecology and Genetics of Microorganisms, Ural Branch, Russian Academy of Sciences, 614081, Perm, Russia.
Perm State National Research University, 614600, Perm, Russia.
出版信息
Dokl Biochem Biophys. 2019 May;484(1):69-72. doi: 10.1134/S1607672919010174. Epub 2019 Apr 22.
The conditions for constructing an immunosorbent reagent for solid-phase NMR analysis were optimized. For this purpose, we increased the area of the sensitized portion of the membrane to fit the relaxometer coil size and added the agent sorption buffer. This provided the penetration of the anti-ligand molecules into the membrane thickness and their uniform distribution.
优化了用于固相核磁共振分析的免疫吸附试剂的构建条件。为此，我们增大了膜致敏部分的面积以适配弛豫仪线圈尺寸，并添加了试剂吸附缓冲液。这使得抗配体分子能够渗透到膜的厚度中并实现均匀分布。
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