Centre for Infectious Diseases Research in Zambia, Lusaka, Zambia.
Department of Biostatistics, School of Public Health, University of Ghana, Accra.
PLoS One. 2020 Jul 1;15(7):e0235322. doi: 10.1371/journal.pone.0235322. eCollection 2020.
The re-emergence of vector borne diseases affecting millions of people in recent years has drawn attention to arboviruses globally. Here, we report on the sero-prevalence of chikungunya virus (CHIKV), dengue virus (DENV), mayaro virus (MAYV) and zika virus (ZIKV) in a swamp community in Zambia.
We collected blood and saliva samples from residents of Lukanga swamps in 2016 during a mass-cholera vaccination campaign. Over 10,000 residents were vaccinated with two doses of Shanchol™ during this period. The biological samples were collected prior to vaccination (baseline) and at specified time points after vaccination. We tested a total of 214 baseline stored serum samples for IgG antibodies against NS1 of DENV and ZIKV and E2 of CHIKV and MAYV on ELISA. We defined sero-prevalence as the proportion of participants with optical density (OD) values above a defined cut-off value, determined using a finite mixture model.
Of the 214 participants, 79 (36.9%; 95% CI 30.5-43.8) were sero-positive for Chikungunya; 23 (10.8%; 95% CI 6.9-15.7) for Zika, 36 (16.8%; 95% CI 12.1-22.5) for Dengue and 42 (19.6%; 95% CI 14.5-25.6) for Mayaro. Older participants were more likely to have Zika virus whilst those involved with fishing activities were at greater risk of contracting Chikungunya virus. Among all the antigens tested, we also found that Chikungunya saliva antibody titres correlated with baseline serum titres (Spearman's correlation coefficient = 0.222; p = 0.03).
Arbovirus transmission is occurring in Zambia. This requires proper screening tools as well as surveillance data to accurately report on disease burden in Zambia.
近年来，影响数百万人的虫媒病毒病再次出现，引起了全球对虫媒病毒的关注。在这里，我们报告了赞比亚一个沼泽社区中基孔肯雅病毒（CHIKV）、登革热病毒（DENV）、马亚罗病毒（MAYV）和寨卡病毒（ZIKV）的血清流行率。
我们在 2016 年大规模霍乱疫苗接种期间，从卢卡nga 沼泽的居民中采集了血液和唾液样本。在此期间，有超过 10000 名居民接种了两剂 Shanchol™。在接种疫苗之前（基线）和接种疫苗后的指定时间点采集了所有生物样本。我们使用有限混合模型确定了一个定义的截止值，对总共 214 个基线储存的血清样本进行了针对 DENV 和 ZIKV 的 NS1 和 CHIKV 和 MAYV 的 E2 的 ELISA 检测，以检测 IgG 抗体。我们将血清流行率定义为 OD 值超过定义截止值的参与者比例。
在 214 名参与者中，有 79 名（36.9%；95%CI 30.5-43.8）对基孔肯雅病毒呈血清阳性；23 名（10.8%；95%CI 6.9-15.7）对寨卡病毒，36 名（16.8%；95%CI 12.1-22.5）对登革热，42 名（19.6%；95%CI 14.5-25.6）对马亚罗病毒。年龄较大的参与者更有可能感染寨卡病毒，而从事捕鱼活动的人感染基孔肯雅病毒的风险更高。在所有测试的抗原中，我们还发现基孔肯雅病毒唾液抗体滴度与基线血清滴度相关（Spearman 相关系数=0.222；p=0.03）。
虫媒病毒在赞比亚传播。这需要适当的筛选工具以及监测数据，以便准确报告赞比亚的疾病负担。
