Garcia John Phillip, Lakshmi Buddolla Anantha, Kim Sanghyo
Department of Bio Nanotechnology, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-Si, Gyeonggi-Do 461-701 Republic of Korea.
3 Biotech. 2019 May;9(5):181. doi: 10.1007/s13205-019-1718-4. Epub 2019 Apr 20.
Ruthenium seems to be a promising alternative to platinum because of the wide range of oxidation states it has and its ability to form complexes with bioactive ligands. In this study, naringin, a naturally occurring flavonoid, was used to synthesize a novel ruthenium complex with potential anticancer activity. The characterization of the synthesized complex was done by UV-Vis spectroscopy, FTIR and NMR studies. In addition, the complex was tested against Human A549 cell lines to determine the anticancer effect, and against human dermal fibroblasts (HDFa) to find any underlying toxicity. Further, the morphological changes of the cancer cells can be determined by using bio-atomic force microscopy. Results showed that the synthesized complex was able to induce anticancer effects against A549 with minimal impact to HDFa. In this study, we investigated the anticancer properties of naringin-ruthenium (II) complex using live- and dead-cell staining assay, MTT, Trypan blue, and lactate dehydrogenase assay. Further, morphological changes were observed in the A549 cells using Bio-AFM. The Bio-AFM results have proven the better cytotoxic behavior of naringin-ruthenium (II) complex. The cell viability results also provided the anticancer efficacy of the complex.
钌似乎是铂的一种有前景的替代物，因为它具有广泛的氧化态，并且能够与生物活性配体形成配合物。在本研究中，柚皮苷（一种天然存在的类黄酮）被用于合成一种具有潜在抗癌活性的新型钌配合物。通过紫外可见光谱、傅里叶变换红外光谱和核磁共振研究对合成的配合物进行了表征。此外，该配合物针对人A549细胞系进行了测试以确定其抗癌效果，并针对人皮肤成纤维细胞（HDFa）进行了测试以发现任何潜在毒性。此外，癌细胞的形态变化可以通过生物原子力显微镜来确定。结果表明，合成的配合物能够对A549诱导抗癌作用，而对HDFa的影响最小。在本研究中，我们使用活细胞和死细胞染色测定、MTT、台盼蓝和乳酸脱氢酶测定研究了柚皮苷-钌（II）配合物的抗癌特性。此外，使用生物原子力显微镜在A549细胞中观察到了形态变化。生物原子力显微镜的结果证明了柚皮苷-钌（II）配合物具有更好的细胞毒性行为。细胞活力结果也提供了该配合物的抗癌功效。
