Key Laboratory of Environmental Pollution and Microecology of Liaoning Province, Shenyang Medical College, No.146, North Huanghe Street, Yuhong District, Shenyang, Liaoning, 110034, China.
Department of Biotechnology, Beijing Center for Physical and Chemical Analysis, No. 27, West Sanhuan North Road, Haidian District, Beijing, 100089, China.
Environ Pollut. 2019 Mar;246:972-979. doi: 10.1016/j.envpol.2018.12.083. Epub 2018 Dec 29.
In order to investigate the relationship between air pollution and the respiratory tract microbiota, 114 healthy volunteers aged 18-21 years were selected during the winter heating period in Northeast China; 35 from a lightly polluted region (group A), 40 from a moderately polluted region (group B) and 39 from a heavily polluted region (group C). Microbial genome DNA was extracted from throat swab samples to study the oral flora composition of the volunteers by amplifying and sequencing the V3 regions of prokaryotic 16S rRNA. Lung function tests were also performed. The relative abundance of Bacteroidetes and Fusobacteria were significantly lower and Firmicutes Proteonacteria and Actinobacteria higher in participants from polluted regions. Within bacteria classes, Bacterioida abundance was lower and Clostridia abundance higher in polluted areas, which was also reflected in the order of abundance. In samples from region C, the abundance of Prevotellaceae, Veillonellaceae, Porphyromonadaceae, Fusobacteriaceae Paraprevollaceae and Flavobacteriaceae were lowest among the 3 regions studied, whereas the abundance of Lachnospiraceae and Ruminococcaceae were the highest. From group A to group C, the relative class abundances of Prevotella, Veillonella, Fusobacterium, Camphylobacter and Capnocytophaga Porphyromonas, Peptostreptococcus and Moraxella became lower in polluted areas. Pulmonary function correlated with air pollution and the oropharyngeal microbiota differed within regions of high, medium and low air pollution. Thus, during the winter heating period in Northeast China, the imbalance of the oropharyngeal microbiota might be caused by air pollution and is likely associated with impairment of lung function in young people.
为了研究空气污染与呼吸道微生物群之间的关系，在中国东北地区冬季供暖期间，选择了 114 名 18-21 岁的健康志愿者；其中 35 名来自轻度污染地区（A 组），40 名来自中度污染地区（B 组），39 名来自重度污染地区（C 组）。从咽喉拭子样本中提取微生物基因组 DNA，通过扩增和测序原核 16S rRNA 的 V3 区来研究志愿者的口腔菌群组成。还进行了肺功能测试。与来自清洁区的志愿者相比，来自污染区的志愿者的拟杆菌门和梭杆菌门的相对丰度明显较低，厚壁菌门、变形菌门和放线菌门的相对丰度较高。在细菌门内，Bacteroidetes 的丰度较低，Clostridia 的丰度较高，这也反映在丰度顺序上。在 C 区的样本中，Prevotellaceae、Veillonellaceae、Porphyromonadaceae、Fusobacteriaceae、Paraprevotellaceae 和 Flavobacteriaceae 的丰度在 3 个研究区域中最低，而 Lachnospiraceae 和 Ruminococcaceae 的丰度最高。从 A 组到 C 组，污染地区的 Prevotella、Veillonella、Fusobacterium、Camphylobacter 和 Capnocytophaga、Porphyromonas、Peptostreptococcus 和 Moraxella 的相对丰度逐渐降低。肺功能与空气污染相关，高、中、低空气污染地区的口咽微生物群存在差异。因此，在中国东北地区冬季供暖期间，口咽微生物群的失衡可能是由空气污染引起的，并且可能与年轻人肺功能受损有关。
