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用于正确测定超氧化物歧化酶的6-羟基多巴胺技术的改进

Modification of the 6-hydroxydopamine technique for the correct determination of superoxide dismutase.

作者信息

Crosti N, Servidei T, Bajer J, Serra A

出版信息

J Clin Chem Clin Biochem. 1987 Apr;25(4):265-6.

PMID:3114411
Abstract

Heikkila & Cabbat (Anal. Biochem. 75, 356-362 (1976] have proposed the autoxidation of the 6-hydroxydopamine as a method to test superoxide dismutase activity in biological samples. This method has several advantages but in some instances leads to incorrect determinations. We present here a necessary modification of the method to avoid bias.

摘要

海基拉和卡巴特（《分析生物化学》第75卷，第356 - 362页，1976年）提出将6 - 羟基多巴胺的自动氧化作为一种检测生物样品中超氧化物歧化酶活性的方法。该方法有几个优点，但在某些情况下会导致测定结果不准确。我们在此提出对该方法进行必要的改进以避免偏差。

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1
Modification of the 6-hydroxydopamine technique for the correct determination of superoxide dismutase.用于正确测定超氧化物歧化酶的6-羟基多巴胺技术的改进
J Clin Chem Clin Biochem. 1987 Apr;25(4):265-6.
2
Effects of superoxide dismutase and catalase on catalysis of 6-hydroxydopamine and 6-aminodopamine autoxidation by iron and ascorbate.超氧化物歧化酶和过氧化氢酶对铁和抗坏血酸催化6-羟基多巴胺和6-氨基多巴胺自氧化的影响。
Biochem Pharmacol. 1981 Aug 15;30(16):2279-85. doi: 10.1016/0006-2952(81)90099-x.
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6-Hydroxydopamine releases iron from ferritin and promotes ferritin-dependent lipid peroxidation.6-羟基多巴胺从铁蛋白中释放铁，并促进铁蛋白依赖性脂质过氧化。
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Effect of ceruloplasmin on 6-hydroxydopamine oxidation.铜蓝蛋白对6-羟基多巴胺氧化的影响。
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Superoxide dismutase and catalase of calf trabecular meshwork.小牛小梁网的超氧化物歧化酶和过氧化氢酶
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[Quantitative method of determining the activity of multiple forms of superoxide dismutase].[超氧化物歧化酶多种形式活性的定量测定方法]
Biull Eksp Biol Med. 1977 Jun;83(6):762-4.
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[The oxidation of superoxide dismutase by hypochlorite. The occurrence of isomers having catalytic activity].[超氧化物歧化酶被次氯酸盐氧化。具有催化活性的异构体的出现]
Dokl Akad Nauk SSSR. 1990;314(6):1500-2.
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Interactions between metals, ligands, and oxygen in the autoxidation of 6-hydroxydopamine: mechanisms by which metal chelation enhances inhibition by superoxide dismutase.6-羟基多巴胺自氧化过程中金属、配体与氧之间的相互作用：金属螯合增强超氧化物歧化酶抑制作用的机制。
Arch Biochem Biophys. 1987 Dec;259(2):305-15. doi: 10.1016/0003-9861(87)90497-8.
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[A simple and sensitive method of determination of superoxide dismutase activity based on the reaction of quercetin oxidation].[一种基于槲皮素氧化反应的简单灵敏的超氧化物歧化酶活性测定方法]
Vopr Med Khim. 1990 Mar-Apr;36(2):88-91.
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6-hydroxydopamine does not reduce molecular oxygen directly, but requires a coreductant.
Arch Biochem Biophys. 1984 May 15;231(1):164-8. doi: 10.1016/0003-9861(84)90373-4.

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