Vo Van Kiet Senior High School, Ho Chi Minh City 700000, Vietnam.
Faculty of Natural Sciences, Thu Dau Mot University, Thu Dau Mot City 820000, Binh Duong province, Vietnam.
Biomolecules. 2019 May 30;9(6):211. doi: 10.3390/biom9060211.
Wild bitter melon ( L. var. Abbreviata Ser.) is a wild edible variety of , often used in folk medicine. In this study, the biological activities of its extract and fractions were investigated in vitro. It was found that ethyl acetate (EA) fraction exhibited high 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging activity with a half maximal inhibitory concentration (IC) value of 0.43 ± 0.04 mg/mL, while the chloroform (CF), EA, and n-butanol (Bu) fractions had strong 2,2-azinobis-3-ethyl benzothiazoline-6-sulfonic acid (ABTS) scavenging ability with IC values of 0.36 ± 0.04 mg/mL, 0.35 ± 0.02 mg/mL, and 0.35 ± 0.05 mg/mL, respectively. Moreover, the EA and Bu fractions exhibited the highest protective effect against HO-induced DNA damage in a concentration-dependent manner. Furthermore, the EA fraction was effective in the inhibition of enzyme α-amylase activity with an IC value of 0.27 ± 0.029 mg/mL. Finally, it was observed that the production of nitric oxide (NO), a pro-inflammatory mediator, was significantly reduced from LPS-stimulated murine macrophage RAW 264.7 cells by the ethanol extract (ET) and the EA fraction. Therefore, wild bitter melon could be considered as a promising biomaterial for the development of pharmaceutical products.
野生苦瓜（L. var. Abbreviata Ser.）是苦瓜的一种野生可食用品种，常用于民间医学。在这项研究中，研究了其提取物和馏分的体外生物活性。结果发现，乙酸乙酯（EA）馏分具有较高的 1,1-二苯基-2-苦基肼（DPPH）清除活性，半最大抑制浓度（IC）值为 0.43±0.04mg/mL，而氯仿（CF）、EA 和正丁醇（Bu）馏分具有较强的 2,2-偶氮双-3-乙基苯并噻唑啉-6-磺酸（ABTS）清除能力，IC 值分别为 0.36±0.04mg/mL、0.35±0.02mg/mL 和 0.35±0.05mg/mL。此外，EA 和 Bu 馏分以浓度依赖的方式对 HO 诱导的 DNA 损伤表现出最强的保护作用。此外，EA 馏分对α-淀粉酶活性具有抑制作用，IC 值为 0.27±0.029mg/mL。最后，观察到乙醇提取物（ET）和 EA 馏分可显著抑制 LPS 刺激的鼠巨噬细胞 RAW 264.7 细胞中促炎介质一氧化氮（NO）的产生。因此，野生苦瓜可被视为开发药物产品的有前途的生物材料。
