酿酒酵母中鸟苷酸对腺苷酸环化酶的激活作用受CDC25起始基因产物的控制。
The activation of adenylate cyclase by guanyl nucleotides in Saccharomyces cerevisiae is controlled by the CDC25 start gene product.
作者信息
Daniel J, Becker J M, Enari E, Levitzki A
机构信息
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
出版信息
Mol Cell Biol. 1987 Oct;7(10):3857-61. doi: 10.1128/mcb.7.10.3857-3861.1987.
Abstract
In the thermosensitive cdc25 start mutant of Saccharomyces cerevisiae, the regulation of adenylate cyclase by guanyl nucleotides was rapidly nullified when the enzyme was prepared from nonsynchronized cells shifted to the restrictive temperature. In agreement with previous in vivo complementation studies, this biochemical defect was fully suppressed by the expression of either the whole cloned CDC25 gene or its C-terminal portion. Moreover, membranes prepared from cdc25(Ts) cells grown at the permissive temperature evinced an altered regulation of adenylate cyclase by guanyl nucleotides. These results indicate that the CDC25 protein, together with RAS, is involved in the regulation of adenylate cyclase by guanyl nucleotides and raise the possibility that adenylate cyclase might form a ternary complex with RAS and CDC25.
摘要
在酿酒酵母的温度敏感型cdc25起始突变体中，当从转移到限制温度的非同步细胞中制备腺苷酸环化酶时，鸟苷酸对腺苷酸环化酶的调节作用迅速消失。与之前的体内互补研究一致，完整的克隆CDC25基因或其C末端部分的表达完全抑制了这种生化缺陷。此外，从在允许温度下生长的cdc25（Ts）细胞制备的膜显示出鸟苷酸对腺苷酸环化酶的调节作用发生了改变。这些结果表明，CDC25蛋白与RAS一起参与了鸟苷酸对腺苷酸环化酶的调节，并增加了腺苷酸环化酶可能与RAS和CDC25形成三元复合物的可能性。
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