Reparto di Sicurezza Chimica degli Alimenti - Istituto Superiore di Sanità, 00161 Rome, Italy.
Dipartimento di Chimica - Università "Sapienza", 00185 Rome, Italy.
Toxins (Basel). 2019 Jun 18;11(6):351. doi: 10.3390/toxins11060351.
Mycotoxins exposure by inhalation and/or dermal contact is possible in different branches of industry especially where heavily dusty settings are present and the handling of dusty commodities is performed. This study aims to explore the validity of the biomonitoring as a tool to investigate the intake of mycotoxins in a population of workers operating in an Italian feed plant. Serum samples were collected for the determination of aflatoxins B1 (AFB1), AFB1-Lysine adduct and ochratoxin A (OTA). A method based on liquid-liquid extraction coupled with high resolution mass spectrometry determination was developed and fully validated. For AFB1, a high number of non-detected samples (90%) was found and no statistical difference was observed comparing workers and control group. None of the analyzed samples showed the presence of AFB1-Lysine adduct. For OTA, the 100% of the analyzed samples was positive with a 33% of the samples showing a concentration higher than the limit of quantification (LOQ), but no statistical difference was highlighted between the average levels of exposed and control groups. In conclusion, the presence of AFB1 and OTA in serum cannot be attributable to occupational exposure.
通过吸入和/或皮肤接触接触霉菌毒素的情况可能发生在不同的行业中，特别是在存在大量粉尘的环境中，以及进行粉尘商品处理的情况下。本研究旨在探索生物监测作为一种工具的有效性，以研究在意大利饲料厂工作的工人人群中摄入霉菌毒素的情况。采集血清样本以测定黄曲霉毒素 B1 (AFB1)、AFB1-赖氨酸加合物和赭曲霉毒素 A (OTA)。开发了一种基于液液萃取结合高分辨率质谱测定的方法，并对其进行了全面验证。对于 AFB1，发现大量未检出的样本（90%），且工人组和对照组之间无统计学差异。未检测到 AFB1-赖氨酸加合物。对于 OTA，100%的分析样本呈阳性，33%的样本浓度高于定量限（LOQ），但暴露组和对照组的平均水平之间没有显著差异。总之，血清中 AFB1 和 OTA 的存在不能归因于职业暴露。
