Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin, 150030, People's Republic of China.
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People's Republic of China.
Antonie Van Leeuwenhoek. 2019 Nov;112(11):1611-1621. doi: 10.1007/s10482-019-01287-3. Epub 2019 Jun 20.
A Gram-stain positive, aerobic, rod-shaped bacterium, designated strain NEAU-85, was isolated from rhizosphere soil of lettuce and characterised using a polyphasic approach. Strain NEAU-85 was found to be catalase positive, motile and able to grow at between 10 and 30 °C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-85 belongs to the genus Cryobacterium and is closely related to the type strain Cryobacterium psychrotolerans JCM 13925 (98.5%) and also to Leifsonia kafniensis NCCB 100216 (97.6%). Multilocus sequence analysis using the concatenated gene sequences of the atpD, dnaK, recA, rpoB, sevY and ychF genes showed that strain NEAU-85 belongs to the genus Cryobacterium. The digital DNA-DNA hybridization values revealed that strain NEAU-85 is distinct from its close phylogenetic neighbour C. psychrotolerans. The menaquinones were identified as MK-10 and MK-11. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phospholipid, an unidentified glycolipid and an unidentified lipid. The major fatty acids were identified as anteiso-C, anteiso-C and iso-C. The genomic DNA G + C content of strain NEAU-85 was determined to be 68.9 mol%. The DNA-DNA hybridization value between them was less than 70%. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-85 can be concluded to represent a novel species of the genus Cryobacterium, for which the name Cryobacterium tepidiphilum sp. nov. is proposed. The type strain is NEAU-85 (= CCTCC AA 2018035 = JCM 32545).
一株革兰氏阳性、需氧、杆状细菌，被命名为 NEAU-85 菌株，从生菜根际土壤中分离出来，并通过多相方法进行了特征描述。NEAU-85 菌株被发现过氧化氢酶阳性、运动和能够在 10 到 30°C 之间生长。基于 16S rRNA 基因序列的系统发育分析表明，NEAU-85 菌株属于 Cryobacterium 属，与模式菌株 Cryobacterium psychrotolerans JCM 13925（98.5%）和 Leifsonia kafniensis NCCB 100216（97.6%）最为密切相关。使用 atpD、dnaK、recA、rpoB、sevY 和 ychF 基因的串联基因序列进行的多位点序列分析表明，NEAU-85 菌株属于 Cryobacterium 属。数字 DNA-DNA 杂交值表明，NEAU-85 菌株与亲缘关系最近的种 Cryobacterium psychrotolerans 不同。menaquinones 被鉴定为 MK-10 和 MK-11。磷脂图谱由双磷脂酰甘油、磷脂酰甘油、磷脂、一种未鉴定的糖脂和一种未鉴定的脂组成。主要脂肪酸为 anteiso-C、anteiso-C 和 iso-C。NEAU-85 菌株的基因组 DNA G+C 含量为 68.9 mol%。它们之间的 DNA-DNA 杂交值小于 70%。基于表型、基因型和系统发育数据，NEAU-85 菌株可被归类为 Cryobacterium 属的一个新种，建议将其命名为 Cryobacterium tepidiphilum sp. nov.。模式菌株为 NEAU-85（= CCTCC AA 2018035 = JCM 32545）。
