Pang G, Wilson J D
Immunology. 1978 Aug;35(2):407-14.
The capacity of purified lymphocytes from human peripheral blood to bind the Fc portion of IgG was investigated by the rosette technique using ox erythrocytes sensitized with rabbit anti-ox IgG (EA) and human erythrocytes sensitized with anti-CD IgG (EA). With unfractionated lymphocytes EA always detected more rosette-forming cells (RFC) than did EA; however, in lymphocyte populations specifically depleted of B lymphocytes by passage through copolymer styrene bead columns, the proportion of rosettes formed with EA and with EA was the same. Double labelling for Fc receptors and surface immunoglobulin (SIg) demonstrated that most of the lymphocytes which formed rosettes with either EA or EA also carried SIg. Pre-incubation of lymphocytes at 37° to remove heatlabile SIg did not affect their ability to form EA rosettes but reduced the proportion of SIg-bearing cells. Following pre-incubation a significant proportion of EA RFC still remained SIg positive but the lymphocytes which formed rosettes with EA no longer carried SIg. These studies suggest that rosette formation with EA detects only K' lymphocytes (non-T, non-B cells bearing heat-labile SIg) while EA detects some B lymphocytes as well. By reducing the amount of IgG bound to ox erythrocytes sensitivity was reduced to the point where EA no longer formed rosettes with B lymphocytes but still detected K' lymphocytes indicating either a qualitative or quantitative difference between the Fc receptors on B and K' lymphocytes. Treatment of lymphocytes with trypsin decreased the percentage of rosettes formed with EA but not with EA supporting the conclusion that there is a structural difference between the Fc receptors on B and K' lymphocytes although a difference in receptor density is not excluded. When EA and fluorescein-labelled ox erythrocytes sensitized with a low concentration of rabbit anti-ox IgG were mixed, most RFC bound both the indicator erythrocytes simultaneously suggesting that the Fc receptors on `K' lymphocytes do not exhibit species specificity.
采用兔抗牛IgG（EA）致敏的牛红细胞和抗人CD IgG（EA）致敏的人红细胞，通过玫瑰花结技术研究了人外周血纯化淋巴细胞结合IgG Fc段的能力。对于未分离的淋巴细胞，EA总是比EA检测到更多的玫瑰花结形成细胞（RFC）；然而，在通过共聚聚苯乙烯珠柱特异性去除B淋巴细胞的淋巴细胞群体中，与EA和EA形成的玫瑰花结比例相同。对Fc受体和表面免疫球蛋白（SIg）进行双重标记表明，大多数与EA或EA形成玫瑰花结的淋巴细胞也携带SIg。将淋巴细胞在37℃预孵育以去除热不稳定的SIg，并不影响它们形成EA玫瑰花结的能力，但降低了携带SIg细胞的比例。预孵育后，相当一部分EA RFC仍然SIg阳性，但与EA形成玫瑰花结的淋巴细胞不再携带SIg。这些研究表明，与EA形成玫瑰花结仅检测到“K”淋巴细胞（携带热不稳定SIg的非T、非B细胞），而EA还检测到一些B淋巴细胞。通过减少与牛红细胞结合的IgG量，敏感性降低到EA不再与B淋巴细胞形成玫瑰花结但仍能检测到“K”淋巴细胞的程度，这表明B淋巴细胞和“K”淋巴细胞上的Fc受体在质量或数量上存在差异。用胰蛋白酶处理淋巴细胞降低了与EA形成的玫瑰花结百分比，但不影响与EA形成的玫瑰花结百分比，这支持了B淋巴细胞和“K”淋巴细胞上的Fc受体存在结构差异这一结论，尽管不排除受体密度存在差异。当将EA与用低浓度兔抗牛IgG致敏的荧光素标记的牛红细胞混合时，大多数RFC同时结合指示红细胞，这表明“K”淋巴细胞上的Fc受体不表现出种属特异性。
