Equine Clinic, National Veterinary School of Toulouse, France.
Department of Histopathology, National Veterinary School of Toulouse, France.
Bioelectrochemistry. 2019 Oct;129:251-258. doi: 10.1016/j.bioelechem.2019.05.018. Epub 2019 Jun 14.
Electrochemotherapy (ECT) is an anticancer bioelectrochemical therapy where electrical field pulses (electropermeabilization) increase intracellular concentration of antitumor drugs. The procedure is very effective against skin tumors. The restrictive regulations concerning anticancer drugs in veterinary medicine limit use of ECT. Electroporation with calcium (Electroporation Calcium Therapy)(ECaT) was proved to be effective in vivo on induced tumors in laboratory animals. This study evaluated the effects of ECaT in equine sarcoids (spontaneous skin tumors) on an animal cohort. Pulse parameters for ECaT were choosen for using skin contact electrodes. ECaT was applied under general anesthesia. The tumors were removed at different days after the treatment and analyzed by histology. The study assessed the volume fraction of necrosis that was >50% for 9 of 13 sarcoids. Sixteen sarcoids in 10 horses were treated with ECaT. Macroscopic changes (a crust) were observed in 14/16 tumors. The main microscopic changes were necrosis, ulceration,hemorrhages, calcifications and thrombosis. The adverse effect was an inflammatory local reaction. Surrounding tissues were not affected. This targeted effect can be explained by its control by the field distribution in the tissue and on the interstitial diffusion of the injected Ca.
电化学疗法（ECT）是一种抗癌生物电化学疗法，其中电场脉冲（电穿孔）增加了抗肿瘤药物的细胞内浓度。该程序对皮肤肿瘤非常有效。兽医中关于抗癌药物的限制性规定限制了 ECT 的使用。已经证明电穿孔用钙（电穿孔钙疗法）（ECaT）在实验室动物的诱导肿瘤中具有体内疗效。本研究在动物队列中评估了 ECaT 在马肉瘤（自发性皮肤肿瘤）中的作用。选择了用于皮肤接触电极的 ECaT 的脉冲参数。在全身麻醉下进行 ECaT 治疗。在治疗后不同天数切除肿瘤并进行组织学分析。该研究评估了 13 个肉瘤中有 9 个的坏死体积分数> 50%。用 ECaT 治疗了 10 匹马的 16 个肉瘤。14/16 个肿瘤观察到宏观变化（结痂）。主要的微观变化是坏死、溃疡、出血、钙化和血栓形成。不良影响是炎症局部反应。周围组织不受影响。这种靶向效应可以通过其在组织中的场分布和注入的 Ca 的间质扩散来控制来解释。
