Frandsen Stine K, Gehl Julie, Tramm Trine, Thoefner Martin S
Center for Experimental Drug and Gene Electrotransfer (C*EDGE), Department of Clinical Oncology and Palliative Care, Zealand University Hospital, Sygehusvej 10, 4000 Roskilde, Denmark.
Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Blegdamsvej 3B, 2200 Copenhagen N, Denmark.
Animals (Basel). 2020 Mar 19;10(3):517. doi: 10.3390/ani10030517.
Sarcoids are common equine skin tumors where the risk of recurrence after treatment is high, and better treatment options are warranted. Calcium electroporation is a novel anti-cancer treatment where lethally high calcium concentrations are introduced into the cells by electroporation, a method where short high-voltage pulses induce transient permeabilization of the cell membrane. This study investigated the safety and long-term response of calcium electroporation on sarcoids. Thirty-two sarcoids in eight horses were included. The study suggested that calcium electroporation is a safe and feasible treatment for sarcoids, including inoperable sarcoids. Horses were treated once (2/8) or twice (6/8) under general anesthesia, where sarcoids were injected with 220 mM calcium chloride followed by electroporation with 8 pulses of 100 μs, 1 kV/cm, and 1 Hz. Biopsies were taken prior to treatment. The sarcoid size was monitored for 12-38 weeks after the first treatment. Complete response was observed in 22% (6/27) of treated sarcoids, and partial response in 22% (6/27), giving a 44% total response. Treatment efficacy did not appear to be related to location, type, or size. In all non-biopsied lesions, a complete response was seen (4/4). In conclusion, in this small study, 44% of sarcoids responded with 22% of sarcoids disappearing.
肉样瘤是常见的马皮肤肿瘤，治疗后复发风险高，因此需要更好的治疗选择。钙电穿孔是一种新型抗癌治疗方法，通过电穿孔将致死性高浓度的钙引入细胞，电穿孔是一种利用短高压脉冲诱导细胞膜瞬时通透化的方法。本研究调查了钙电穿孔治疗肉样瘤的安全性和长期疗效。纳入了8匹马身上的32个肉样瘤。研究表明，钙电穿孔是一种治疗肉样瘤（包括无法手术切除的肉样瘤）的安全可行的方法。马匹在全身麻醉下接受一次（2/8）或两次（6/8）治疗，向肉样瘤内注射220 mM氯化钙，然后用8个脉冲进行电穿孔，脉冲参数为100 μs、1 kV/cm和1 Hz。在治疗前进行活检。在首次治疗后对肉样瘤大小进行了12 - 38周的监测。在22%（6/27）的治疗肉样瘤中观察到完全缓解，22%（6/27）为部分缓解，总缓解率为44%。治疗效果似乎与位置、类型或大小无关。在所有未活检的病变中，均观察到完全缓解（4/4）。总之，在这项小型研究中，44%的肉样瘤有反应，其中22%的肉样瘤消失。
