Laboratório de Química Orgânica, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
ICBAS-Instituto de Ciências Biomédicas Abel Salazar, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
Mar Drugs. 2019 Jun 25;17(6):379. doi: 10.3390/md17060379.
Previously unreported meroterpene, acremine S (), and benzopyran derivative, acremine T (), were isolated, together with lumichrome (), ergosterol () and ergosterol 5,8-endoperoxide, from cultures of the marine sponge-associated fungus KUF1007. The structure of the previously unreported compounds was established based on an extensive analysis of 1D and 2D NMR spectra as well as HRMS data. The absolute configurations of the stereogenic centers of were established, unambiguously, based on NOESY correlations and comparison of calculated and experimental electronic circular dichroism (ECD) spectra. Compounds were tested for their acetylcholinesterase and butyrylcholinesterase inhibitory activities.
先前未报道的混合萜烯 acremine S () 和苯并吡喃衍生物 acremine T ()，与光氨酸 ()、麦角甾醇 () 和麦角甾醇 5,8-内过氧化物一起，从海洋海绵相关真菌 KUF1007 的培养物中分离得到。先前未报道的化合物的结构是基于对 1D 和 2D NMR 光谱以及高分辨率质谱 (HRMS) 数据的广泛分析确定的。基于 NOESY 相关和计算与实验电子圆二色性 (ECD) 光谱的比较，明确确定了的立体中心的绝对构型。测试了化合物对乙酰胆碱酯酶和丁酰胆碱酯酶的抑制活性。
