Cooper C, Jones H G, Weller R O, Walker V
J Neurol Neurosurg Psychiatry. 1984 Jun;47(6):579-84. doi: 10.1136/jnnp.47.6.579.
Tumour cell-rich platelet-free preparations were isolated from 21 fresh samples of human intracranial tumours using enzymic digestion, followed by discontinuous density gradient centrifugation on Percoll and (14 preparations) adherence on plastic. Of the disaggregated cells 79.8 to 97.7% (mean 86.2%) were tumour cells, and mean cell viability was 82.6%. All the tumours produced prostaglandin (PG), E2, F2 alpha, 6 oxo F1 alpha and Thromboxane B2 during 16 hours of incubation but the amount varied widely. Highest production of PGE2 and TXB2 per 10(5) cells was by the eight meningiomas in which the prostanoid profile closely resembled that of circulating monocytes.
使用酶消化法从21份新鲜人类颅内肿瘤样本中分离出富含肿瘤细胞的无血小板制剂，随后在Percoll上进行不连续密度梯度离心，并对14份制剂进行塑料贴壁处理。在解离的细胞中，79.8%至97.7%（平均86.2%）为肿瘤细胞，平均细胞活力为82.6%。所有肿瘤在孵育16小时期间均产生前列腺素（PG）、E2、F2α、6-氧代F1α和血栓素B2，但产量差异很大。每10(5)个细胞中PGE2和TXB2产量最高的是8例脑膜瘤，其类前列腺素谱与循环单核细胞的非常相似。
