China MOA Laboratory for Risk Assessment of Quality and Safety of Livestock and Poultry Products, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Quality Operation Laboratory at University of Veterinary and Animal Sciences, Lahore, 54600, Pakistan.
Ann Clin Microbiol Antimicrob. 2019 Jul 5;18(1):21. doi: 10.1186/s12941-019-0317-x.
The development of antibiotic resistance in bacteria is a major public health threat. Infection rates of resistant pathogens continue to rise against nearly all antimicrobials, which has led to development of different strategies to combat the antimicrobial resistance. In this review, we discuss how the newly popular CRISPR-cas system has been applied to combat antibiotic resistance in both extracellular and intracellular pathogens. We also review a recently developed method in which nano-size CRISPR complex was used without any phage to target the mecA gene. However, there is still challenge to practice these methods in field against emerging antimicrobial resistant pathogens.
细菌对抗生素耐药性的发展是一个主要的公共卫生威胁。耐药病原体的感染率继续上升，几乎所有的抗菌药物都受到了影响，这导致了不同的策略来对抗抗菌药物耐药性。在这篇综述中，我们讨论了新流行的 CRISPR-cas 系统如何应用于对抗细胞外和细胞内病原体的抗生素耐药性。我们还回顾了一种最近开发的方法，即使用纳米级 CRISPR 复合物，而无需任何噬菌体来靶向 mecA 基因。然而，在实践中，这些方法仍然面临着针对新兴抗菌药物耐药性病原体的挑战。
Zotero 插件