Ząbek T, Semik-Gurgul E, Szmatoła T, Gurgul A, Fornal A, Bugno-Poniewierska M
National Research Institute of Animal Production, Balice 32-083, Poland.
University Centre of Veterinary Medicine, University of Agriculture in Kraków, Al. Mickiewicza 24/28, 30-059 Kraków, Poland.
Int J Genomics. 2019 Jun 2;2019:2839614. doi: 10.1155/2019/2839614. eCollection 2019.
Horses are one of the longest-living species of farm animals. Advanced age is often associated with a decrease in body condition, dysfunction of immune system, and late-onset disorders. Due to this, the search for new solutions in the prevention and treatment of pathological conditions of the advanced age of horses is desirable. That is why the identification of aging-related changes in the horse genome is interesting in this respect. In the recent years, the research on aging includes studies of age-related epigenetic effects observed on the DNA methylation level. We applied reduced representation bisulfite sequencing (RRBS) to uncover a range of age DMR sites in genomes of blood leukocytes derived from juvenile and aged horses of native Hucul breed. Genes colocated with age-related differentially methylated regions (age DMRs) are the members of pathways involved in cellular signal transduction, immune response, neurogenesis, differentiation, development, and cancer progression. A positive correlation was found between methylation states and gene expression in particular loci from our data set. Some of described age DMR-linked genes were also reported elsewhere. Obtained results contribute to the knowledge about the molecular basis of aging of equine blood cells.
马是寿命最长的农场动物物种之一。老龄通常与身体状况下降、免疫系统功能障碍和迟发性疾病有关。因此，寻求预防和治疗老龄马病理状况的新解决方案是很有必要的。正因如此，在这方面，识别马基因组中与衰老相关的变化很有意思。近年来，衰老研究包括对DNA甲基化水平上观察到的与年龄相关的表观遗传效应的研究。我们应用简化代表性亚硫酸氢盐测序（RRBS）来揭示来自本地胡库尔品种幼年和老龄马的血液白细胞基因组中的一系列年龄差异甲基化区域（DMR）位点。与年龄相关的差异甲基化区域（年龄DMR）共定位的基因是参与细胞信号转导、免疫反应、神经发生、分化、发育和癌症进展的途径的成员。从我们的数据集中发现甲基化状态与特定基因座中的基因表达之间存在正相关。一些所述的与年龄DMR相关的基因在其他地方也有报道。获得的结果有助于了解马血细胞衰老的分子基础。
