Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, 210093, Jiangsu Province, China.
Center for Translational Medicine and Jiangsu Key Laboratory of Molecular Medicine, Medical School of Nanjing University, Nanjing, 210093, Jiangsu Province, China.
Life Sci. 2019 Sep 1;232:116647. doi: 10.1016/j.lfs.2019.116647. Epub 2019 Jul 10.
Brain injury after sepsis leads to high mortality and long-term brain dysfunction in patients. Previous studies revealed that borneol has a protective effect on the brain, but its function on sepsis associated encephalopathy (SAE) remains unknown. Herein, we investigated the protective effect of borneol against sepsis-related brain injury.
Lipopolysaccharide (LPS)-induced sepsis mice and cells were treated with borneol at the dose of 100 mg/kg by gavage or 10 μg/ml in culture, respectively. The protective effect of borneol on neurons and the microglia were assessed in vivo and in vitro.
We observed that borneol attenuated brain neuronal and microglial inflammation in LPS-induced sepsis mice with a suppression of p-p65 and p38 signaling that were initially activated by LPS in the brain. In vitro examination confirmed that the protective effect of borneol on both neurons and microglia, and its suppressive effect on p-p65 and p38 pathways were, at least in part, direct.
An early protection of neurons and microglia from bacterial endotoxin during sepsis is beneficial, and borneol has the potential to protect these cells.
脓毒症后的脑损伤导致患者死亡率高和长期脑功能障碍。先前的研究表明，冰片对大脑具有保护作用，但它在脓毒症相关性脑病（SAE）中的作用尚不清楚。在此，我们研究了冰片对脓毒症相关脑损伤的保护作用。
通过灌胃或培养中分别以 100mg/kg 和 10μg/ml 的剂量用冰片处理脂多糖（LPS）诱导的脓毒症小鼠和细胞。在体内和体外评估了冰片对神经元和小胶质细胞的保护作用。
我们观察到，冰片减轻了 LPS 诱导的脓毒症小鼠的脑神经元和小胶质细胞炎症，抑制了 LPS 在脑中最初激活的 p-p65 和 p38 信号通路。体外研究证实，冰片对神经元和小胶质细胞的保护作用及其对 p-p65 和 p38 通路的抑制作用至少部分是直接的。
脓毒症期间对细菌内毒素的早期保护神经元和小胶质细胞是有益的，冰片具有保护这些细胞的潜力。
