School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, P. R. China.
Chem Commun (Camb). 2019 Aug 14;55(63):9311-9314. doi: 10.1039/c9cc04077c. Epub 2019 Jul 16.
Discrimination between cysteine and homocysteine at the single-molecule level is achieved within a K238Q mutant aerolysin nanopore, which provides a confined space for high spatial resolution to identify the amino acid difference with a 5'-benzaldehyde poly(dA) probe. Our strategy allows potential detection and characterization of various amino acids and their modifications, and provides a crucial step towards developing nanopore protein sequencing devices.
在 K238Q 突变 Aerolysin 纳米孔内实现了半胱氨酸和高半胱氨酸的单分子水平区分，该纳米孔为高空间分辨率识别与 5'-苯甲醛聚（dA）探针的氨基酸差异提供了受限空间。我们的策略允许对各种氨基酸及其修饰进行潜在的检测和表征，并为开发纳米孔蛋白质测序设备提供了关键步骤。
