Isolation, characterization, and molecular identification of species from urinary tract infections.

BACKGROUND AND PURPOSE

species are reportedly the most common human fungal pathogens. The incidence of urinary tract infections (UTIs) caused by pathogens has increased in recent decades. However, such infections rarely occur in the absence of any predisposing factors. Regarding this, the aim of the present study was to identify the species causing UTIs and determine the predisposing factors for candiduria.

MATERIALS AND METHODS

The current study was conducted on 1,450 urine samples obtained from patients suspected of UTI. Out of this number, 19 cases were candidiasis, and 2 cases were mixed infections caused by bacteria and fungi. species were diagnosed differentially using the germ tube test, colony staining on CHROMagar medium, intracellular beta-glucosidase enzyme activity, and glucose absorption pattern. Then, the colonies with the same morphology were confirmed by the DNA sequencing of internal transcribed spacer regions.

RESULTS

According to the results, 38%, 28.6%, 14.3%, and 9.5% of the isolates were identified as and / respectively. The presence of one or more predisposing factors was proved in all patients in whom diabetes was the most prevalent predisposing factor (21.1%).

CONCLUSION

Based on the obtained results, species was the most prevalent fungal species. In addition, urinary fungal infections were less prevalent than bacterial urinary infections.