Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Joint Laboratory of Guangdong Province and Hong Kong Region on Marine Bioresource Conservation and Exploitation, College of Marine Sciences, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2019 Jul 14;20(14):3459. doi: 10.3390/ijms20143459.
is the principal pathogen causing blue mold of citrus. Searching for novel antifungal agents is an important aspect of the post-harvest citrus industry because of the lack of higher effective and low toxic antifungal agents. Herein, the effects of 2-methoxy-1,4-naphthoquinone (MNQ) on and its mechanism were carried out by a series of methods. MNQ had a significant anti- effect with an MIC value of 5.0 µg/mL. The label-free protein profiling under different MNQ conditions identified a total of 3037 proteins in the control group and the treatment group. Among them, there were 129 differentially expressed proteins (DEPs, up-regulated > 2.0-fold or down-regulated < 0.5-fold, < 0.05), 19 up-regulated proteins, 26 down-regulated proteins, and 67 proteins that were specific for the treatment group and another 17 proteins that were specific for the control group. Of these, 83 proteins were sub-categorized into 23 hierarchically-structured GO classifications. Most of the identified DEPs were involved in molecular function (47%), meanwhile 27% DEPs were involved in the cellular component and 26% DEPs were involved in the biological process. Twenty-eight proteins identified for differential metabolic pathways by KEGG were sub-categorized into 60 classifications. Functional characterization by GO and KEGG enrichment results suggests that the DEPs are mainly related to energy generation (mitochondrial carrier protein, glycoside hydrolase, acyl-CoA dehydrogenase, and ribulose-phosphate 3-epimerase), NADPH supply (enolase, pyruvate carboxylase), oxidative stress (catalase, glutathione synthetase), and pentose phosphate pathway (ribulose-phosphate 3-epimerase and xylulose 5-phosphate). Three of the down-regulated proteins selected randomly the nitro-reductase family protein, mono-oxygenase, and cytochrome P450 were verified using parallel reaction monitoring. These findings illustrated that MNQ may inhibit by disrupting the metabolic processes, especially in energy metabolism and stimulus response that are both critical for the growth of the fungus. In conclusion, based on the molecular mechanisms, MNQ can be developed as a potential anti-fungi agent against .
是引起柑橘青霉病的主要病原体。由于缺乏高效低毒的抗真菌剂，寻找新型抗真菌剂是柑橘采后产业的一个重要方面。在此，通过一系列方法研究了 2-甲氧基-1,4-萘醌（MNQ）对 的作用及其机制。MNQ 具有显著的抗真菌作用，其 MIC 值为 5.0 µg/mL。在不同 MNQ 条件下的无标记蛋白质谱分析共鉴定出对照组和处理组中的 3037 种蛋白质。其中，有 129 种差异表达蛋白（DEPs，上调>2.0 倍或下调<0.5 倍， < 0.05），19 种上调蛋白，26 种下调蛋白，67 种处理组特有的蛋白和另外 17 种对照组特有的蛋白。其中，83 种蛋白质被分为 23 个层次结构的 GO 分类。大多数鉴定的 DEPs 参与分子功能（47%），同时 27%的 DEPs 参与细胞成分，26%的 DEPs 参与生物过程。通过 KEGG 鉴定的 28 种差异代谢途径蛋白被分为 60 个分类。GO 和 KEGG 富集结果的功能特征表明，DEPs 主要与能量产生（线粒体载体蛋白、糖苷水解酶、酰基辅酶 A 脱氢酶和核酮糖-1,5-二磷酸 3-差向异构酶）、NADPH 供应（烯醇酶、丙酮酸羧化酶）、氧化应激（过氧化氢酶、谷胱甘肽合酶）和戊糖磷酸途径（核酮糖-1,5-二磷酸 3-差向异构酶和木酮糖 5-磷酸）有关。随机选择的三种下调蛋白，即硝基还原酶家族蛋白、单加氧酶和细胞色素 P450，使用平行反应监测进行了验证。这些发现表明，MNQ 可能通过破坏代谢过程来抑制 ，特别是在对真菌生长至关重要的能量代谢和刺激反应中。总之，根据分子机制，MNQ 可以开发为一种针对 的潜在抗真菌剂。
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