Department of Spine and Spinal Cord Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, No. 7 Weiwu road, 450003, Zhengzhou, P. R. China.
Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Jiefang Avenue 1095, 430030, Wuhan, P. R. China.
J Mater Sci Mater Med. 2019 Jul 24;30(8):89. doi: 10.1007/s10856-019-6289-8.
As a non-invasive biophysical therapy, electromagnetic fields (EMF) have been widely used to promote the healing of fractures. In the present study, hydroxyapatite/collagen I (HAC) loaded with rabbit bone marrow mesenchymal stem cells (MSCs) were cultured in a dynamic perfusion bioreactor and exposed to EMF of 15 Hz/1mT. Osteogenic differentiation of the seeded cells was analyzed through the evaluation of ALP activity and osteogenesis-related genes expression in vitro. The in vivo osteogenesis efficacy of the cell laden HAC constructs treated with/without EMF was evaluated through a rabbit femur condyle defect model. The results showed that EMF of 15 Hz/1mT could enhance the osteogenic differentiation of the cells seeded on HAC scaffold. Furthermore, the in vivo experiments demonstrated that EMF exposure could promote bone regeneration within the defect and bone integration between the graft and host bone. Taking together, the MSCs seeded HAC scaffold combined with EMF exposure could be a promising approach for bone tissue engineering.
作为一种非侵入性的生物物理疗法，电磁场（EMF）已被广泛用于促进骨折愈合。在本研究中，将负载有兔骨髓间充质干细胞（MSCs）的羟基磷灰石/胶原 I（HAC）在动态灌注生物反应器中培养，并暴露于 15 Hz/1 mT 的 EMF 中。通过体外评估 ALP 活性和骨形成相关基因的表达来分析接种细胞的成骨分化。通过兔股骨髁缺损模型评估了经/未经 EMF 处理的负载细胞的 HAC 构建体的体内成骨功效。结果表明，15 Hz/1 mT 的 EMF 可以增强接种在 HAC 支架上的细胞的成骨分化。此外，体内实验表明，EMF 暴露可以促进缺损内的骨再生和移植物与宿主骨之间的骨整合。综上所述，MSCs 接种的 HAC 支架结合 EMF 暴露可能是一种有前途的骨组织工程方法。
