College of Landscape Architecture, Central South University of Forestry and Technology, Changsha, 410004, Hunan, China.
College of Landscape Architecture, Central South University of Forestry and Technology, Changsha, 410004, Hunan, China.
Plant Physiol Biochem. 2019 Sep;142:312-331. doi: 10.1016/j.plaphy.2019.07.002. Epub 2019 Jul 2.
Heat stress limits the growth of roses and adversely affects the yield and the quality of the rose cut-flowers. To investigate the heat stress response (HSR) mechanisms of rose, we compared the transcriptome profiling generated from Rosa chinensis 'Slater's crimson China' exposed to heat stress for five different time duration (0, 0.5, 2, 6, 12 h). Overall, 6175 differentially expressed genes (DGEs) were identified and exhibited different temporal expression patterns. Up-regulated genes related to chaperone-mediated protein folding, signal transduction and ROS scavenging were rapidly induced after 0.5-2 h of heat treatment, which provides evidence for the early adjustments of heat stress response in R. chinensis. While the down-regulated genes related to light reaction, sucrose biosynthesis, starch biosynthesis and cell wall biosynthesis were identified after as short as 6 h of heat stress, which indicated the ongoing negative effects on the physiology of R. chinensis. Using weighted gene co-expression network analysis, we found that different heat stress stages could be delineated by several modules. Based on integrating the transcription factors with upstream enriched DNA motifs of co-expressed genes in these modules, the gene regulation networks were predicted and several regulators of HSR were identified. Of particular importance was the discovery of the module associated with rapid sensing and signal transduction, in which numerous co-expressed genes related to chaperones, Ca signaling pathways and transcription factors were identified. The results of this study provided an important resource for further dissecting the role of candidate genes governing the transcriptional regulatory network of HSR in Rose.
高温胁迫限制了玫瑰的生长，对玫瑰切花的产量和品质产生不利影响。为了研究玫瑰的高温胁迫响应（HSR）机制，我们比较了暴露于不同高温胁迫时间（0、0.5、2、6 和 12 h）下的香水月季 '斯莱特的猩红中国' 的转录组谱。总的来说，鉴定了 6175 个差异表达基因（DGE），并表现出不同的时间表达模式。在 0.5-2 h 的热处理后，快速诱导与伴侣介导的蛋白质折叠、信号转导和 ROS 清除相关的上调基因，这为香水月季的 HSR 早期调整提供了证据。而在 6 h 的热胁迫后，鉴定到与光反应、蔗糖生物合成、淀粉生物合成和细胞壁生物合成相关的下调基因，这表明对香水月季的生理功能产生了持续的负面影响。通过加权基因共表达网络分析，我们发现几个模块可以划分不同的高温胁迫阶段。基于将转录因子与这些模块中共同表达基因的上游富集 DNA 基序整合，预测了基因调控网络，并鉴定了几个 HSR 的调节剂。特别重要的是发现了与快速感应和信号转导相关的模块，其中鉴定到了大量与伴侣蛋白、Ca 信号通路和转录因子相关的共同表达基因。本研究的结果为进一步剖析候选基因在调控玫瑰 HSR 转录调控网络中的作用提供了重要资源。
