Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, China.
School of Food Engineering, Tianjin Tianshi College, Tianjin, China.
J Food Biochem. 2019 Jul;43(7):e12851. doi: 10.1111/jfbc.12851. Epub 2019 Apr 14.
Rosemary ethanol extract (REE) from Rosmarinus officinalis was identified by LC-ESI-MS/MS and 12 compounds were found. Among them, rosmarinic acid (389.78 μg/mg in REE), luteolin-3'-O-glucuronide (325.58 μg/mg), luteolin-5-O-glucuronide (120.92 μg/mg), and geniposide (120.83 μg/mg) are the major components. The antioxidant activity evaluation of REE by off-line HPLC methods indicated that among the 12 compounds, rosmarinic acid had the strongest scavenging activities in both DPPH· and ·OH. The cytotoxicity experiment showed that REE with the concentration ranges from 1 to 100 µg/ml did not significantly affect the cell viability of HeLa, while inhibitory rate reduced to 62.3% when the concentration was increased to 1,000 µg/ml. The results of intracellular antioxidation assay showed that the ability of REE in reducing the reactive oxygen species (ROS) in HeLa cells was higher than rosmanol, and lower than rosmarinic acid without cell toxicity. PRACTICAL APPLICATIONS: Plant polyphenols are essential components of functional foods, due to their antioxidant and enzyme inhibition activities. This paper is the first study about the quantification of antioxidant compounds, antioxidant activity evaluation, and their cellular antioxidant activity of polyphenols extract from R. officinalis toward HeLa cells. We aimed to elucidate the chemical composition and recognition of antioxidant components with DPPH and OH free radicals scavenging activity. In addition, the polyphenols dose-response correlations with cellular antioxidant activity were also determined. These results indicated that off-line HPLC method with DPPH and OH free radicals as markers is available for screening antioxidant activity of polyphenols from the mixture.
迷迭香乙醇提取物（REE）从迷迭香中被鉴定出来。通过 LC-ESI-MS/MS 发现了 12 种化合物。其中，迷迭香酸（REE 中的 389.78μg/mg）、木樨草素-3'-O-葡萄糖苷（325.58μg/mg）、木樨草素-5-O-葡萄糖苷（120.92μg/mg）和栀子苷（120.83μg/mg）是主要成分。通过离线 HPLC 方法对 REE 的抗氧化活性评价表明，在这 12 种化合物中，迷迭香酸在 DPPH·和·OH 中均具有最强的清除活性。细胞毒性实验表明，浓度范围在 1 至 100μg/ml 的 REE 对 HeLa 细胞的活力没有显著影响，而当浓度增加到 1000μg/ml 时，抑制率降低到 62.3%。细胞内抗氧化测定结果表明，REE 降低 HeLa 细胞中活性氧（ROS）的能力高于罗莎醇，低于无细胞毒性的迷迭香酸。实际应用：植物多酚是功能性食品的重要组成部分，因为它们具有抗氧化和酶抑制活性。本文首次研究了迷迭香中多酚提取物对 HeLa 细胞的抗氧化化合物定量、抗氧化活性评价及其细胞抗氧化活性。我们旨在阐明具有 DPPH 和 OH 自由基清除活性的抗氧化成分的化学组成和识别。此外，还确定了多酚与细胞抗氧化活性的剂量反应关系。这些结果表明，使用 DPPH 和 OH 自由基作为标志物的离线 HPLC 方法可用于筛选多酚混合物的抗氧化活性。
