Área de Microbiología, Departamento de Ciencias de la Salud, Facultad de Ciencias Experimentales, Universidad de Jaén, 23071, Jaén, Spain.
Centre of Water, Environment, Sustainability and Public Health; Department of Civil and Environmental Engineering, University of Strathclyde, Glasgow, Scotland, United Kingdom.
Sci Rep. 2019 Jul 29;9(1):10938. doi: 10.1038/s41598-019-47384-1.
In silico analysis of Lactobacillus pentosus MP-10 plasmids (pLPE-1 to pLPE-5) suggests that plasmid-borne genes mediate the persistence of lactobacilli during olive fermentation and enhance their probiotic properties and their competitiveness in several ecological niches. The role of plasmids in the probiotic activities of L. pentosus MP-10 was investigated by plasmid-curing process which showed that plasmids contribute in increased metal tolerance and the biosequestration of several metals such as iron, aluminium, cobalt, copper, zinc, cadmium and mercury. Statistically significant differences in mucin adhesion were detected between the uncured and the cured L. pentosus MP-10, which possibly relied on a serine-rich adhesin (sraP) gene detected on the pLPE-2 plasmid. However, plasmid curing did not affect their tolerance to gastro-intestinal conditions, neither their growth ability under pre-determined conditions, nor auto-aggregation and pathogen co-aggregation were changed among the cured and uncured L. pentosus MP-10. These findings suggest that L. pentosus MP-10 plasmids play an important role in gastro-intestinal protection due to their attachment to mucin and, thus, preventing several diseases. Furthermore, L. pentosus MP-10 could be used as a bioquencher of metals in the gut, reducing the amount of these potentially toxic elements in humans and animals, food matrices, and environmental bioremediation.
戊糖片球菌 MP-10 质粒（pLPE-1 至 pLPE-5）的计算机分析表明，质粒携带的基因介导了乳酸菌在橄榄发酵过程中的持久性，并增强了它们的益生菌特性和在多个生态位中的竞争力。通过质粒消除过程研究了质粒对戊糖片球菌 MP-10 益生菌活性的作用，结果表明质粒有助于提高金属耐受性和几种金属（如铁、铝、钴、铜、锌、镉和汞）的生物固定。未消除和消除的戊糖片球菌 MP-10 之间在粘蛋白粘附方面存在统计学上的显著差异，这可能依赖于 pLPE-2 质粒上检测到的富含丝氨酸的粘附素（sraP）基因。然而，质粒消除并不影响它们对胃肠条件的耐受性，也不影响它们在预定条件下的生长能力，消除和未消除的戊糖片球菌 MP-10 之间的自聚集和病原体共聚集也没有改变。这些发现表明，由于戊糖片球菌 MP-10 质粒与粘蛋白结合，因此在胃肠道保护中发挥重要作用，并可预防多种疾病。此外，戊糖片球菌 MP-10 可用作肠道金属的生物清除剂，减少人类和动物、食品基质和环境生物修复中这些潜在有毒元素的含量。
