提取物通过数字X射线摄影术对Wistar大鼠骨密度的影响。

Effect of extract on bone mineral density in Wistar rats via digital radiography.

作者信息

Arbabzadegan Nazanin, Moghadamnia Ali Akbar, Kazemi Sohrab, Nozari Farideh, Moudi Ehsan, Haghanifar Sina

机构信息

Student Research Committee, Babol University of Medical Science, Babol, Iran.

Neuroscience Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

出版信息

Caspian J Intern Med. 2019 Spring;10(2):176-182. doi: 10.22088/cjim.10.2.176.

DOI:10.22088/cjim.10.2.176

PMID:31363396

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6619477/

Abstract

BACKGROUND

Osteoporosis is a common disease of old age. The present study used digital radiography to determine the effects of extract on the bone mineral density (BMD) of experimental rats.

METHODS

In this experimental study, 25 male and 25 female Wistar rats, aged three weeks old and weighing 100 grams, were randomly divided into five groups: (1) control group, (2) calcium/vitamin D group, (3) 60 mg/kg extract group, (4) 90 mg/kg extract group and (5) 120 mg/kg extract group. Rats received these diets for 30 days. The spongy bone density was measured in the maxilla and mandible using digital radiography and the serum levels of calcium, vitamin D and phosphorus were measured at baseline and after 30 days. The data were analyzed using ANOVA and Tukey test.

RESULTS

There was no significant difference between serum calcium and phosphorus levels in the five groups before and after 30 days. The serum vitamin D in the group receiving calcium and vitamin D was significantly higher than in the other groups (with average values of 24.7, 61.7, 23.47, 23.95 and 39.16 in the male groups 1 to 5 and 29.0, 85.07, 31.58, 42.34 and 18.83 in the female groups 1 to 5, respectively (p<0.001). Moreover, the increased mandibular BMD in the 120 mg/kg group was significantly higher than in the control group (p<0.01).

CONCLUSION

A diet containing 120 mg/kg extract resulted in increased mandibular bone mineral density.

摘要

背景

骨质疏松症是一种常见的老年疾病。本研究采用数字X线摄影术来确定提取物对实验大鼠骨密度（BMD）的影响。

方法

在本实验研究中，将25只三周龄、体重100克的雄性Wistar大鼠和25只雌性Wistar大鼠随机分为五组：（1）对照组，（2）钙/维生素D组，（3）60毫克/千克提取物组，（4）90毫克/千克提取物组和（5）120毫克/千克提取物组。大鼠接受这些饮食30天。使用数字X线摄影术测量上颌骨和下颌骨的海绵骨密度，并在基线和30天后测量血清钙、维生素D和磷水平。数据采用方差分析和Tukey检验进行分析。

结果

30天前后，五组大鼠的血清钙和磷水平无显著差异。接受钙和维生素D组的血清维生素D显著高于其他组（雄性组1至5的平均值分别为24.7、61.7、23.47、23.95和39.16，雌性组1至5的平均值分别为29.0、85.07、31.58、42.34和18.83，p<0.001）。此外，120毫克/千克组下颌骨骨密度的增加显著高于对照组（p<0.01）。