Menghini L, Ferrante C, Leporini L, Recinella L, Chiavaroli A, Leone S, Pintore G, Vacca M, Orlando G, Brunetti L
Department of Pharmacy, G. dAnnunzio University, Chieti, Italy.
Department of Chemistry and Pharmacy, University of Sassari, Sassari, Italy.
J Biol Regul Homeost Agents. 2016 Oct-Dec;30(4):985-996.
A pivotal role in osteoporosis development is played by radical oxygen species (ROS), the increased production of which is related to inhibited osteoblastic activity and bone formation. A new field of research could involve medicinal plants with antioxidant and protective effects in osteoporosis. Furthermore, considering the multifactorial metabolic aspects of osteoporosis, the pharmacological association of multiple medicinal plants could improve patient response. The aim of the present study is to evaluate in vitro and in vivo the protective effects of a natural formula containing lactoferrin 12%, Equisetum arvensis ES 54%, soy isoflavones 34% and vitamin D3 0.002%, in PBMC and C2C12 cells and in the bone matrix of young (3-month-old) and aged (12-month-old) female Sprague-Dawley rats, following chronic (21 days) administration. In this context, we assayed the activities of several inflammation and bone homeostasis mediators, such as IL-6, TNFα, PGE2, osteoprotegerin, RANK, RANKL and NFkB. In vitro studies showed that natural formula (5-1000μg/ml) was able to significantly inhibit ROS and PGE2 production. In the same concentration range, the natural formula inhibited both TNFα and IL-6 gene expression. In the in vivo studies, we administered to young and aged female rats the natural formula at 5mg/rat for 21 days, finding a significant reduction in inflammatory PGE2 and NFkB activity. Nevertheless, we observed a significant increase in osteoprotegerin/RANKL ratio only in aged rats, compared to the respective control group. In conclusion, our findings corroborate the rational use of natural formula in the prevention and management of osteoporotic disease.
活性氧（ROS）在骨质疏松症的发展中起着关键作用，其产生增加与成骨细胞活性和骨形成受抑制有关。一个新的研究领域可能涉及对骨质疏松症具有抗氧化和保护作用的药用植物。此外，考虑到骨质疏松症的多因素代谢方面，多种药用植物的药理联合应用可能会改善患者的反应。本研究的目的是在体外和体内评估一种天然配方的保护作用，该配方含有12%的乳铁蛋白、54%的问荆提取物ES、34%的大豆异黄酮和0.002%的维生素D3，在慢性（21天）给药后，对年轻（3个月大）和老年（12个月大）雌性Sprague-Dawley大鼠的外周血单核细胞（PBMC）和C2C12细胞以及骨基质的影响。在此背景下，我们检测了几种炎症和骨稳态介质的活性，如白细胞介素-6（IL-6）、肿瘤坏死因子α（TNFα）、前列腺素E2（PGE2）、骨保护素、核因子κB受体活化因子（RANK）、核因子κB受体活化因子配体（RANKL）和核因子κB（NFkB）。体外研究表明，天然配方（5 - 1000μg/ml）能够显著抑制ROS和PGE2的产生。在相同浓度范围内，天然配方抑制了TNFα和IL-6的基因表达。在体内研究中，我们给年轻和老年雌性大鼠以5mg/只的剂量给予天然配方，持续21天，发现炎症性PGE2和NFkB活性显著降低。然而，与各自的对照组相比，我们仅在老年大鼠中观察到骨保护素/RANKL比值显著增加。总之，我们的研究结果证实了天然配方在骨质疏松症疾病预防和管理中的合理应用。
