Bandekar Siddhesh, Patil Suvarna, Dudulwar Divya, Moogi Prashant Prakash, Ghosh Surabhi, Kshirsagar Shirin
Department of Conservative Dentistry and Endodontics, Yogita Dental College and Hospital, Ratnagiri, Maharashtra, India.
Department of Conservative Dentistry and Endodontics, Vasantdada Patil Dental College and Hospital, Sangli, Maharashtra, India.
J Conserv Dent. 2019 May-Jun;22(3):305-309. doi: 10.4103/JCD.JCD_13_19.
The purpose of this study was to evaluate and compare remineralization potential of fluoride, amorphous calcium phosphate-casein phosphopeptide (ACP-CPP), and combination of hydroxyapatite (HAP) and fluoride on enamel lesions.
Ten intact caries-free human premolars were selected. The coronal portion of each tooth was sectioned into four parts to make four enamel blocks. The baseline surface microhardness (SMH) was measured for all the enamel specimens using Vickers microhardness testing machine. The artificial carious lesion was created by immersing the specimens in demineralizing solution for 3 consecutive days at 35° The SMH of each specimen was evaluated. All the four enamel sections of each tooth were subjected to various surface treatments, i.e., Group A - Fluoride varnish, Group B - ACP-CPP, Group C - Combination of HAP and fluoride (Clinpro), and Group D - Control group; no surface treatment. A carious progress test (pH cycle) was carried out which consisted of alternative demineralization (3 h) and remineralization using artificial saliva (21 h.) for 5 consecutive days. After pH cycling, SMH readings of each specimen were again assessed to evaluate remineralization potential of each surface treatment agent.
Data obtained were statistically analyzed using one-way ANOVA followed by Tukey-Kramer multiple comparison test which was applied to detect significant differences between different surface treatments at different phases of studies.
Fluoride varnish shows higher remineralization potential of early carious lesion compare to ACP-CPP and Clinpro.
本研究旨在评估和比较氟化物、无定形磷酸钙 - 酪蛋白磷酸肽（ACP - CPP）以及羟基磷灰石（HAP）与氟化物组合对牙釉质病变的再矿化潜力。
选取10颗完整无龋的人类前磨牙。将每颗牙齿的冠部切成四部分制成四个牙釉质块。使用维氏显微硬度测试机测量所有牙釉质标本的基线表面显微硬度（SMH）。通过将标本在35°下连续3天浸泡在脱矿溶液中制造人工龋损病变。评估每个标本的SMH。每颗牙齿的所有四个牙釉质切片都进行了各种表面处理，即A组 - 氟化物清漆，B组 - ACP - CPP，C组 - HAP与氟化物组合（Clinpro），D组 - 对照组；不进行表面处理。进行了龋病进展测试（pH循环），包括交替脱矿（3小时）和使用人工唾液再矿化（21小时），连续进行5天。pH循环后，再次评估每个标本的SMH读数，以评估每种表面处理剂的再矿化潜力。
使用单因素方差分析对获得的数据进行统计分析，随后进行Tukey - Kramer多重比较测试，用于检测不同阶段不同表面处理之间的显著差异。
与ACP - CPP和Clinpro相比，氟化物清漆对早期龋损病变显示出更高的再矿化潜力。
