Lasa Rodrigo, Navarro-de-la-Fuente Laura, Gschaedler-Mathis Anne C, Kirchmayr Manuel R, Williams Trevor
Red de Manejo Biorracional de Plagas y Vectores, Instituto de Ecología AC, Xalapa, Veracruz 91070, Mexico.
Facultad de Ciencias Agrícolas, Universidad Veracruzana, Xalapa, Veracruz 91090, Mexico.
Insects. 2019 Jul 31;10(8):228. doi: 10.3390/insects10080228.
Specific ecological interactions between insects and microbes have potential in the development of targeted pest monitoring or control techniques for the spotted wing drosophilid, (Matsumura), an exotic invasive pest of soft fruit. To evaluate attraction to yeast species from preferred types of fruit, three yeasts were isolated from blackberry fruit and two yeasts from raspberry fruit and used to bait simple plastic bottle traps. and were identified from blackberries, whereas a different strain was identified from raspberry. Yeast identification was based on sequence analysis of the D1/D2 domain of the large subunit 26S rRNA gene. Commercial baker's yeast () was similar or more effective for the capture of males and females than yeasts isolated from blackberry or raspberry when grown in sucrose. However, when grown in corn syrup, a strain of from blackberry captured the highest number of females and a strain of isolated from raspberry captured high numbers of males and females. Species of , , and from a laboratory yeast collection did not outperform baker's yeast in pairwise tests when grown in sucrose solution or yeast-peptone-dextrose medium. The raspberry strain of grown in corn syrup outperformed grown in sucrose, in terms of captures in baited traps under laboratory conditions. We conclude that yeast species, strain, and growth medium can have a marked influence on attraction to baited traps, a finding that could assist in the development of yeast-related monitoring or control techniques targeted at this pest.
昆虫与微生物之间特定的生态相互作用，在开发针对斑翅果蝇（Matsumura）的靶向害虫监测或控制技术方面具有潜力，斑翅果蝇是一种入侵性软果害虫。为了评估对来自偏好水果类型的酵母种类的吸引力，从黑莓果实中分离出三种酵母，从覆盆子果实中分离出两种酵母，并用于诱捕简单的塑料瓶陷阱。从黑莓中鉴定出了[具体名称1]和[具体名称2]，而从覆盆子中鉴定出了一种不同的[具体名称3]菌株。酵母鉴定基于大亚基26S rRNA基因D1/D2结构域的序列分析。当在蔗糖中生长时，商业面包酵母（[具体名称4]）在捕获斑翅果蝇雄性和雌性方面与从黑莓或覆盆子中分离出的酵母相似或更有效。然而，当在玉米糖浆中生长时，从黑莓中分离出的一种[具体名称5]菌株捕获的雌性数量最多，从覆盆子中分离出的一种[具体名称6]菌株捕获了大量的雄性和雌性。在蔗糖溶液或酵母蛋白胨葡萄糖培养基中生长时，实验室酵母菌株库中的[具体名称7]、[具体名称8]和[具体名称9]种类在成对测试中表现不如面包酵母。在实验室条件下，在玉米糖浆中生长的覆盆子[具体名称6]菌株在诱饵陷阱捕获方面比在蔗糖中生长的[具体名称6]表现更好。我们得出结论，酵母种类、菌株和生长培养基对斑翅果蝇对诱饵陷阱的吸引力有显著影响，这一发现有助于开发针对这种害虫的与酵母相关的监测或控制技术。
