Swartz R P, Naai D, Vogel C W, Yeager H
Department of Medicine, Georgetown University Medical Center, Washington, D.C. 20007.
Infect Immun. 1988 Sep;56(9):2223-7. doi: 10.1128/iai.56.9.2223-2227.1988.
We investigated the influence of serum factors on the uptake of various species of mycobacteria by human peripheral blood monocytes (PBM). On the basis of the percentage of PBM involved during in vitro uptake, the mycobacteria were of two distinct groups. The mycobacteria of one group, which consisted of Mycobacterium avium complex and M. chelonae, were taken up by many PBM; the other group, consisting of M. tuberculosis, M. kansasii, M. fortuitum, and M. gordonae, were taken up by fewer PBM. M. scrofulaceum was intermediate to these two groups on the basis of its uptake by PBM. Serum depleted of complement by heating or treatment with cobra venom factor significantly reduced the extent of PBM involvement with M. avium complex, indicating that complement is an important serum component mediating the uptake of M. avium complex organisms. Preincubation of mycobacteria with serum containing 10 mM EGTA [ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid] and 10 mM MgCl2 resulted in uptake by a high percentage of PBM, while preincubation in heated serum or serum containing 10 mM EDTA resulted in a significantly reduced percentage of PBM involved in uptake of M. avium complex organisms, indicating that these organisms are activators of the alternative pathway of complement. Incubation of M. avium complex organisms in human serum consumed 51% of the hemolytic complement activity. Parallel experiments indicated that serum had a lesser effect on the uptake of M. tuberculosis. Thus, serum is important in in vitro M. avium complex uptake by PBM; complement has a major role in the effect of serum, but this role is less important with M. tuberculosis.
我们研究了血清因子对人外周血单核细胞（PBM）摄取各种分枝杆菌的影响。根据体外摄取过程中参与的PBM百分比，分枝杆菌分为两个不同的组。一组分枝杆菌由鸟分枝杆菌复合体和龟分枝杆菌组成，被许多PBM摄取；另一组由结核分枝杆菌、堪萨斯分枝杆菌、偶然分枝杆菌和戈登分枝杆菌组成，被较少的PBM摄取。根据PBM对瘰疬分枝杆菌的摄取情况，它介于这两组之间。通过加热或用眼镜蛇毒因子处理使补体缺失的血清显著降低了PBM对鸟分枝杆菌复合体的摄取程度，表明补体是介导鸟分枝杆菌复合体摄取的重要血清成分。将分枝杆菌与含有10 mM乙二醇双（β-氨基乙基醚）-N，N，N'，N'-四乙酸（EGTA）和10 mM氯化镁的血清预孵育，导致高百分比的PBM摄取，而在加热血清或含有10 mM乙二胺四乙酸（EDTA）的血清中预孵育则导致参与摄取鸟分枝杆菌复合体的PBM百分比显著降低，表明这些生物体是补体替代途径的激活剂。将鸟分枝杆菌复合体在人血清中孵育消耗了51%的溶血补体活性。平行实验表明血清对结核分枝杆菌摄取的影响较小。因此，血清在体外PBM摄取鸟分枝杆菌复合体中很重要；补体在血清的作用中起主要作用，但对结核分枝杆菌来说，这种作用不太重要。
