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从[具体来源未给出]中鉴定出的AabHLH35转录因子参与耐寒和耐旱过程。

The AabHLH35 Transcription Factor Identified from is Involved in Cold and Drought Tolerance.

作者信息

Jiang Li, Tian Xingkai, Li Shuting, Fu Yanxia, Xu Jiaojun, Wang Guangdong

机构信息

Department of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Plants (Basel). 2019 Jul 11;8(7):216. doi: 10.3390/plants8070216.

Abstract

Lind. is a popular potted and cut-flower plant with an attractive spathe and foliage. It is native to tropical rainforest areas and is able to blossom throughout the year under suitable conditions. However, various abiotic stresses seriously restrict the ornamental value of and increase the costs of cultivation. A () leaf color mutant of 'Sonate', which accumulates high levels of anthocyanin, has shown increased vigor and tolerance to stresses during cultivation and is, thus, an ideal germplasm for studying stress tolerance in this species. Here, we show that the anthocyanin content in mutant plants at different stages of leaf development was higher than in wild-type (WT) plants, and the ability to tolerate under low-temperature (LT, 14 °C) stress was stronger in than in WT plants. RNA-Seq of cDNA libraries from young leaves of and WT identified as a differentially expressed gene (DEG) that was significantly up-regulated in . Furthermore, heterologous expression of improved tolerance to cold and drought stresses in . These results have built an important molecular foundation for further study of stress tolerance in .

摘要

Lind.是一种受欢迎的盆栽和切花植物,具有吸引人的佛焰苞和叶片。它原产于热带雨林地区,在适宜条件下能够全年开花。然而,各种非生物胁迫严重限制了其观赏价值并增加了种植成本。‘Sonate’的一个花青素含量高的叶色突变体,在栽培过程中表现出更强的活力和胁迫耐受性,因此是研究该物种胁迫耐受性的理想种质。在此,我们表明,突变体植物在叶片发育不同阶段的花青素含量高于野生型(WT)植物,并且在低温(LT,14°C)胁迫下,突变体比WT植物具有更强的耐受性。对突变体和WT幼叶的cDNA文库进行RNA测序,鉴定出一个差异表达基因(DEG),该基因在突变体中显著上调。此外,该基因的异源表达提高了突变体对寒冷和干旱胁迫的耐受性。这些结果为进一步研究该物种的胁迫耐受性奠定了重要的分子基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d1bc/6681207/7c5828a46170/plants-08-00216-g001.jpg

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