Sarfraz Muhammad Hassan, Zubair Muhammad, Aslam Bilal, Ashraf Asma, Siddique Muhammad Hussnain, Hayat Sumreen, Cruz Jorrdy Neves, Muzammil Saima, Khurshid Mohsin, Sarfraz Muhammad Farrukh, Hashem Abeer, Dawoud Turki M, Avila-Quezada Graciela Dolores, Abd Allah Elsayed Fathi
Institute of Microbiology, Government College University, Faisalabad, Pakistan.
Department of Bioinformatics and Biotechnology, Government College University, Faisalabad, Pakistan.
Front Microbiol. 2023 May 31;14:1188743. doi: 10.3389/fmicb.2023.1188743. eCollection 2023.
The aim of this study was to provide a comparative analysis of chitosan (CH), copper oxide (CuO), and chitosan-based copper oxide (CH-CuO) nanoparticles for their application in the healthcare sector. The nanoparticles were synthesized by a green approach using the extract of . The synthesized nanoparticles were characterized using different techniques, such as the synthesis of the particles, which was confirmed by UV-visible spectrometry that showed absorbance at 300 nm, 255 nm, and 275 nm for the CH, CuO, and CH-CuO nanoparticles, respectively. The spherical morphology of the nanoparticles and the presence of active functional groups was validated by SEM, TEM, and FTIR analysis. The crystalline nature of the particles was verified by XRD spectrum, and the average crystallite sizes of 33.54 nm, 20.13 nm, and 24.14 nm were obtained, respectively. The characterized nanoparticles were evaluated for their antibacterial and antibiofilm potential against isolates, where potent activities were exhibited by the nanoparticles. The bioassay for antioxidant activity also confirmed DPPH scavenging activity for all the nanoparticles. This study also evaluated anticancer activities of the CH, CuO, and CH-CuO nanoparticles against HepG2 cell lines, where maximum inhibitions of 54, 75, and 84% were recorded, respectively. The anticancer activity was also confirmed by phase contrast microscopy, where the treated cells exhibited deformed morphologies. This study demonstrates the potential of the CH-CuO nanoparticle as an effective antibacterial agent, having with its antibiofilm activity, and in cancer treatment.
本研究的目的是对壳聚糖（CH）、氧化铜（CuO）和壳聚糖基氧化铜（CH-CuO）纳米颗粒在医疗保健领域的应用进行比较分析。这些纳米颗粒采用绿色方法，使用[提取物名称]提取物合成。通过不同技术对合成的纳米颗粒进行表征，例如通过紫外可见光谱法确认颗粒的合成，该光谱显示CH、CuO和CH-CuO纳米颗粒在300nm、255nm和275nm处有吸光度。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）和傅里叶变换红外光谱（FTIR）分析验证了纳米颗粒的球形形态和活性官能团的存在。通过X射线衍射（XRD）光谱验证了颗粒的晶体性质，分别获得了33.54nm、20.13nm和24.14nm的平均微晶尺寸。对表征后的纳米颗粒针对[分离株名称]的抗菌和抗生物膜潜力进行了评估，纳米颗粒表现出强大的活性。抗氧化活性的生物测定也证实了所有纳米颗粒均具有1,1-二苯基-2-三硝基苯肼（DPPH）清除活性。本研究还评估了CH、CuO和CH-CuO纳米颗粒对肝癌细胞系（HepG2）的抗癌活性，分别记录到最大抑制率为54%、75%和84%。相差显微镜也证实了抗癌活性，其中处理后的细胞呈现出变形的形态。本研究证明了CH-CuO纳米颗粒作为一种有效的抗菌剂，具有抗生物膜活性以及在癌症治疗方面的潜力。
