Laboratório HERCULES, Universidade de Évora, Largo Marquês de Marialva 8, Évora, 7000-809, Portugal; Instituto de Ciências Agrárias e Ambientais Mediterrânicas (ICAAM), Núcleo da Mitra, Apartado 94, Universidade de Évora, Évora, 7006-554, Portugal.
Laboratório HERCULES, Universidade de Évora, Largo Marquês de Marialva 8, Évora, 7000-809, Portugal.
Food Chem Toxicol. 2019 Nov;133:110747. doi: 10.1016/j.fct.2019.110747. Epub 2019 Aug 1.
Three autochthonous flavouring herbs from Alentejo (Portugal), Calamintha nepeta (syn. Clinopodium nepeta), Origanun virens and Thymus mastichina, were selected to evaluate toxicological, antioxidant, antiproliferative and antimicrobial potential of their essential oils (EOs). C. nepeta and T. mastichina EOs showed a high content of oxygenated monoterpenes (86-91%) while O. virens had similar content of oxygenated and hydrocarbon monoterpenes (45%). Toxicological assessment suggests high activity against A. salina (31.8 < CL < 128.4 mg/L) and very low toxicity in Swiss mice (DL ≥ 1500 mg/kg). EOs showed high antioxidant ability by DPPH radical scavenging assay (0.1-0.6 mg QE/mL EO), total reducing power method (0.2-1.7 mg QE/mL EO) and β-carotene/linoleic acid system (11-501 mg QE/mL EO). An important antiproliferative effect against human breast tumour cell line was observed (88.9 < EC < 108.5 mg/L). Moreover, EOs presented a large antibacterial spectrum. Results point out the low toxicity and high antioxidant, antiproliferative and antimicrobial activities of EOs of these endemic aromatic plants, suggesting their potential use in biotechnological, food and/or pharmaceutical industries.
从葡萄牙阿连特茹地区选择了三种土生土长的香料植物——荆芥（Calamintha nepeta，也称为 Clinopodium nepeta）、牛至（Origanun virens）和马郁兰（Thymus mastichina），以评估其精油的毒理学、抗氧化、抗增殖和抗菌潜力。C. nepeta 和 T. mastichina 的精油含有高比例的含氧单萜（86-91%），而 O. virens 的含氧和烃类单萜含量相似（45%）。毒理学评估表明，它们对盐水丰年虾（A. salina）具有高活性（31.8<CL<128.4mg/L），对瑞士小鼠的毒性非常低（DL≥1500mg/kg）。精油通过 DPPH 自由基清除测定（0.1-0.6mg QE/mL 精油）、总还原力法（0.2-1.7mg QE/mL 精油）和β-胡萝卜素/亚油酸体系（11-501mg QE/mL 精油）表现出高抗氧化能力。还观察到对人乳腺癌肿瘤细胞系的重要抗增殖作用（88.9<EC<108.5mg/L）。此外，精油还呈现出广泛的抗菌谱。结果表明，这些地方特有的芳香植物精油具有低毒性、高抗氧化、抗增殖和抗菌活性，提示它们在生物技术、食品和/或制药行业具有潜在的应用价值。
