Liang Yongqi, Xie Min, Li Juan, Liu Liangliang, Cao Yi
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, People's Republic of China.
Key Laboratory of Environment-Friendly Chemistry and Applications of Ministry Education, Laboratory of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan, People's Republic of China.
Dose Response. 2019 Jul 23;17(3):1559325819865713. doi: 10.1177/1559325819865713. eCollection 2019 Jul-Sep.
Polyphenols as typical food components can influence the colloidal properties and internalization of nanomaterials (NMs) into mammalian cells. Recently, we found that 3-hydroxyflavone (H3) promoted intracellular Zn ions in ZnO nanoparticle (NP) exposed Caco-2 and HepG2 cells. However, it is unclear if H3 could affect the internalization of metal-based NMs with different morphologies. This study investigated the influence of H3 on colloidal aspects of Ag NPs and Ag nanoflakes (NFs) as well as the internalization of Ag NMs into THP-1 macrophages. 3-Hydroxyflavone at 50 μM promoted the solubility and altered hydrodynamic size, polydispersity index, and ζ potential of Ag NPs and Ag NFs, which indicated that H3 could affect the colloidal stability of Ag NMs. Only H3 but not Ag NMs significantly decreased mitochondrial activities of THP-1 macrophages. The internalization of Ag NMs was markedly increased due to the presence of H3. 3-Hydroxyflavone also exhibited antioxidative properties as it reduced intracellular reactive oxygen species and promoted the activities of ABC transporters as it reduced retention of Calcein in Ag NM-exposed THP-1 macrophages. We concluded that H3 promoted the internalization of Ag NMs into macrophages probably by altering the colloidal stability of Ag NMs and consequently NM-macrophage interactions.
多酚作为典型的食物成分，可影响纳米材料（NMs）的胶体性质及其进入哺乳动物细胞的内化过程。最近，我们发现3-羟基黄酮（H3）可促进暴露于氧化锌纳米颗粒（NP）的Caco-2和HepG2细胞内的锌离子含量。然而，尚不清楚H3是否会影响不同形态的金属基纳米材料的内化过程。本研究调查了H3对银纳米颗粒（Ag NPs）和银纳米片（NFs）胶体性质的影响，以及银纳米材料进入THP-1巨噬细胞的内化过程。50 μM的3-羟基黄酮可提高Ag NPs和Ag NFs的溶解度，并改变其流体动力学尺寸、多分散指数和ζ电位，这表明H3可影响银纳米材料的胶体稳定性。只有H3而非银纳米材料可显著降低THP-1巨噬细胞的线粒体活性。由于H3的存在，银纳米材料的内化过程明显增加。3-羟基黄酮还表现出抗氧化特性，因为它可减少细胞内活性氧的产生，并通过降低钙黄绿素在暴露于银纳米材料的THP-1巨噬细胞中的潴留来促进ABC转运蛋白的活性。我们得出结论，H3可能通过改变银纳米材料的胶体稳定性，进而改变纳米材料与巨噬细胞的相互作用，促进银纳米材料进入巨噬细胞。
