State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, China.
Marine Biotechnology Research Center, Shandong University, Qingdao, 266237, China.
Antonie Van Leeuwenhoek. 2019 Dec;112(12):1841-1848. doi: 10.1007/s10482-019-01301-8. Epub 2019 Aug 6.
A Gram-stain-negative, aerobic, non-flagellated, rod-shaped bacterium, designated strain SM1703, was isolated from Antarctic seawater collected near the Chinese Antarctic Great Wall Station, King George Island, West Antarctica. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain SM1703 formed a distinct phylogenetic lineage within the family 'Rhodobacteraceae', sharing high 16S rRNA gene sequence similarity with Marivita litorea (95.5%). The strain grew at 10-37 °C (optimum, 25 °C) and with 0.5-13% (w/v) NaCl (optimum, 3-5%). The major cellular fatty acids were C cyclo ω8c, Cω7c, C 2-OH and C 2-OH. The major polar lipids were phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid and an unidentified lipid. The genomic DNA G+C content of strain SM1703 was 64.6 mol%. Based on the results of the polyphasic characterization for strain SM1703, it is classified as the representative of a novel species in a new genus of the family 'Rhodobacteraceae', for which the name Chachezhania antarctica gen. nov., sp. nov. is proposed. The type strain of Chachezhania antarctica is SM1703 (= MCCC 1K03470 = KCTC 62794 = CCTCC AB 2018351).
一株革兰氏阴性、需氧、不具鞭毛的杆状细菌，被命名为 SM1703 菌株，从中国南极长城站附近的南极海水样本中分离得到，位于南极洲的乔治王岛西部。基于 16S rRNA 基因序列的系统发育分析表明，菌株 SM1703 形成了 Rhodobacteraceae 科内的一个独特的进化分支，与 Marivita litorea 的 16S rRNA 基因序列相似度最高（95.5%）。该菌株在 10-37°C（最适温度 25°C）和 0.5-13%（w/v）NaCl（最适盐度 3-5%）的条件下生长。主要的细胞脂肪酸为 C cyclo ω8c、Cω7c、C 2-OH 和 C 2-OH。主要的极性脂类为磷脂酰甘油、磷脂酰胆碱、一种未鉴定的胺脂和一种未鉴定的脂类。菌株 SM1703 的基因组 DNA G+C 含量为 64.6 mol%。基于对 SM1703 菌株的多相特征研究，将其分类为 Rhodobacteraceae 科中一个新属的代表新种，命名为 Chachezhania antarctica gen. nov.，sp. nov.。该种的模式菌株为 SM1703（= MCCC 1K03470 = KCTC 62794 = CCTCC AB 2018351）。
