Biospectrum Life Science Institute, Yongin, Korea.
Skincure Life Science Institute, Yongin, Korea.
J Cosmet Dermatol. 2020 Apr;19(4):977-984. doi: 10.1111/jocd.13086. Epub 2019 Aug 7.
Protocatechuic acid has reported containing antioxidant effects. However, information on its other biological activities such as anti-wrinkle properties is limited AIMS: The objective of this study was to evaluate an antioxidant, collagen synthesis, MMP-1 inhibition (in vitro), and anti-wrinkle (in vivo) effects of protocatechuic acid (PCA) as a potent ingredient for wrinkle-care cosmetic.
Antioxidant effect was evaluated based on its scavenging activity for free radicals (DPPH, ABTS+). To evaluate the anti-skin aging potency of PCA, levels of MMP-1 and type I procollagen were measured using an ELISA kit in cultured human dermal fibroblasts. To further investigate if PCA could increase collagen synthesis, full-thickness human skin explants were immunostained with an anti-collagen I antibody. In an in vivo study, 22 female subjects were enrolled in a placebo-controlled trial. Facial wrinkle, especially crow's feet around eyes, was treated with lotion-containing 0.02% PCA for 8 weeks and compared with the placebo.
In in vitro study, PCA showed high antioxidant activ ity. PCA also showed potential to induce the synthesis of type I collagen in human dermal fibroblast and skin explants. It inhibited MMP-1 secretion from UVA-irradiated human dermal fibroblast. An in vivo study, treatment with lotion-containing 0.02% PCA for 8 weeks significantly reduced the percentage of all skin wrinkle parameters.
Based on the results of in vitro assays and in vivo skin testing in human subjects, PCA shows potential in anti-wrinkle or anti-skin aging treatments.
原儿茶酸具有抗氧化作用。然而，关于其其他生物活性，如抗皱特性的信息有限。
本研究旨在评估原儿茶酸（PCA）作为一种有效的抗皱化妆品成分的抗氧化、胶原合成、MMP-1 抑制（体外）和抗皱（体内）作用。
根据其对自由基（DPPH、ABTS+）的清除活性评估抗氧化作用。为了评估 PCA 的抗皮肤衰老作用，使用 ELISA 试剂盒测量培养的人真皮成纤维细胞中 MMP-1 和 I 型前胶原的水平。为了进一步研究 PCA 是否可以增加胶原合成，使用抗胶原 I 抗体对全厚人皮肤标本进行免疫染色。在体内研究中，22 名女性受试者参加了一项安慰剂对照试验。使用含有 0.02% PCA 的乳液治疗面部皱纹，特别是眼睛周围的鱼尾纹，并与安慰剂进行比较。
在体外研究中，PCA 表现出高抗氧化活性。PCA 还显示出诱导人真皮成纤维细胞和皮肤标本中 I 型胶原合成的潜力。它抑制了 UVA 照射的人真皮成纤维细胞中 MMP-1 的分泌。一项体内研究表明，使用含有 0.02% PCA 的乳液治疗 8 周可显著减少所有皮肤皱纹参数的百分比。
基于体外测定和人体皮肤测试的结果，PCA 在抗皱或抗皮肤衰老治疗方面具有潜力。
