一种新型B淋巴细胞激活剂——内毒素蛋白的化学和物理性质表征
Characterization of the chemical and physical properties of a novel B-lymphocyte activator, endotoxin protein.
作者信息
Goodman G W, Sultzer B M
出版信息
Infect Immun. 1979 Jun;24(3):685-96. doi: 10.1128/iai.24.3.685-696.1979.
Abstract
Endotoxin protein, a novel mouse B-lymphocyte mitogen, is a hydrophobic acidic compound composed of approximately 85% protein and 2.2% glucosamine, but no 2-keto-3-deoxyoctonate. Endotoxin protein also contains lipid, and analysis of the fatty acids in this material demonstrated the presence of beta-hydroxymyristate, a marker for lipid A. In addition, analysis of endotoxin protein by polyacrylamide gel electrophoresis showed that it is heterogeneous, containing four or five major polypeptides, depending upon the bacterial species from which it was isolated. The mitogenicity of endotoxin protein was diminished by alkaline hydrolysis, but not by treatment with hydrochloric or acetic acid. Furthermore, its activity was resistant to digestion with trypsin, chymotrypsin, and pronase and was only partially degraded by papain.
摘要
内毒素蛋白是一种新型小鼠B淋巴细胞有丝分裂原，是一种疏水性酸性化合物，约由85%的蛋白质和2.2%的氨基葡萄糖组成，但不含2-酮-3-脱氧辛酸。内毒素蛋白还含有脂质，对该物质中的脂肪酸分析表明存在β-羟基肉豆蔻酸，这是脂多糖A的标志物。此外，通过聚丙烯酰胺凝胶电泳对内毒素蛋白进行分析表明它是异质性的，包含四或五条主要多肽，这取决于从中分离它的细菌种类。内毒素蛋白的促有丝分裂活性可通过碱性水解而降低，但盐酸或乙酸处理则不会。此外，其活性对胰蛋白酶、胰凝乳蛋白酶和链霉蛋白酶的消化具有抗性，仅被木瓜蛋白酶部分降解。
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