Hsu S M, Hsu P L, Lo S S, Wu K K
Department of Pathology, University of Texas Health Science Center, Houston 77225.
Am J Pathol. 1988 Oct;133(1):5-12.
The synthesis of prostaglandin, prostacyclin, and thromboxane, which requires the enzyme prostaglandin H (PGH) synthase (cyclooxygenase), is a general property of histiocytes, monocytes, and Langerhans cells. Previously the authors reported the production of prostaglandin E2 in a Hodgkin's cell line, KM-H2, and suggested that these cells therefore have a functional similarity to histiocyte-related cells. The present study confirms that the Hodgkin's neoplastic (Reed-Sternberg, H-RS) cells in tissue are also capable of producing prostaglandins by demonstrating the presence of PGH synthase in these cells in B5-fixed, paraffin-embedded tissue sections. It was found that the H-RS cells from seven of ten patients with Hodgkin's disease were stained variously with anti-PGH synthase antibodies. In normal and reactive lymphoid tissues, anti-PGH synthase staining was restricted to histiocytes, endothelial cells, and interdigitating reticulum cells. Thus, this study provides further evidence for a possible relationship between H-RS cells and histiocytes or interdigitating reticulum cells; this relationship has also been supported by information obtained in extensive immunologic, biochemical, and cell-differentiation studies. The secretion of PGH-synthase products, especially prostaglandin E2, in H-RS cells may play a major role in the regulation of cellular immunity in patients with Hodgkin's disease.
前列腺素、前列环素和血栓素的合成需要前列腺素H（PGH）合酶（环氧化酶），这是组织细胞、单核细胞和朗格汉斯细胞的共同特性。此前，作者报道了霍奇金细胞系KM-H2中前列腺素E2的产生，并提出这些细胞因此与组织细胞相关细胞具有功能相似性。本研究通过在B5固定、石蜡包埋的组织切片中证明这些细胞中存在PGH合酶，证实了组织中的霍奇金肿瘤性（里德-施特恩伯格，H-RS）细胞也能够产生前列腺素。研究发现，10例霍奇金病患者中有7例的H-RS细胞被抗PGH合酶抗体不同程度地染色。在正常和反应性淋巴组织中，抗PGH合酶染色仅限于组织细胞、内皮细胞和交错突网状细胞。因此，本研究为H-RS细胞与组织细胞或交错突网状细胞之间可能存在的关系提供了进一步证据；广泛的免疫学、生化和细胞分化研究获得的信息也支持了这种关系。H-RS细胞中PGH合酶产物的分泌，尤其是前列腺素E2，可能在霍奇金病患者的细胞免疫调节中起主要作用。
